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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Apolipoprotein A I/APOA1 Antibody Picoband®

SKU : BHA21000817

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Mfr.No.	PB9916, PB9916-10ug, PB9916-Biotin, PB9916-Cy3, PB9916-Dylight488, PB9916-Dylight550, PB9916-Dylight594, PB9916-FITC, PB9916-HRP, PB9916-APC, PB9916-PE, PB9916-iFluor647, PB9916-carrier-free
Alternative Names	Apolipoprotein A-I
Background	Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Apolipoprotein A I/APOA1 Antibody Picoband® catalog # PB9916. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activat
Host	Rabbit
Immunogen Region	E. coli-derived human APOA1 recombinant protein (Position: D25-Q267). Human APOA1 shares 64% and 61.7% amino acid (aa) sequence identity with mouse and rat APOA1, respectively.
Isotype	Rabbit IgG
Molecular Weight	26 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Apolipoprotein A-I;Apo-AI;ApoA-I;Apolipoprotein A1;Proapolipoprotein A-I;ProapoA-I;Truncated apolipoprotein A-I;Apolipoprotein A-I (1-242);APOA1;
Tissue Specificity	Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. .
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P02647
Gene Id	335
Gene Name	APOA1
Subcellular Location	Secreted.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IHC, ICC, WB
Description	Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat ELISA , 0.1-0.5ug/ml, Human, - Weste blot, 0.1-0.5ug/ml, Human Immunohistochemistry (Frozen Section), 0.5-1ug/ml, Human Immunocytochemistry, 0.5-1ug/ml, Human Flow Cytometry (Fixed), 1-3ug/1x10⁶ cells, Human

Reference	1. Brewer, H. B., Jr.; Fairwell, T.; LaRue, A.; Ronan, R.; Houser, A.; Bronzert, T. J. : The amino acid sequence of human apoA-I, an apolipoprotein isolated from high absorbance lipoproteins. Biochem. Biophys. Res. Commun. 80: 623-630, 1978. 2. Ma J, Liao XL, Lou B, Wu MP (2004). "Role of apolipoprotein A-I in protecting against endotoxin toxicity". Acta Biochim. Biophys. Sin. (Shanghai) 36 (6): 41924. 3. Yui Y, Aoyama T, Morishita H, Takahashi M, Takatsu Y, Kawai C (1988). "Serum prostacyclin stabilizing factor is identical to apolipoprotein A-I (Apo A-I). A novel function of Apo A-I". J. Clin. Invest. 82 (3): 8037. 4. Zhang, Y.; Zanotti, I.; Reilly, M. P.; Glick, J. M.; Rothblat, G. H.; Rader, D. J. : Overexpression of apolipoprotein A-I promotes reverse transport of cholesterol from macrophages to feces in vivo. Circulation 108: 661-663, 2003.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000817-1	PB9916	100 ug	-	$370		Add to Cart
BHA21000817-2	PB9916-10ug	10 ug	Sample	$99		Add to Cart
BHA21000817-3	PB9916-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000817-4	PB9916-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000817-5	PB9916-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000817-6	PB9916-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000817-7	PB9916-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000817-8	PB9916-FITC	100 ug	FITC	$570		Add to Cart
BHA21000817-9	PB9916-HRP	100 ug	HRP	$570		Add to Cart
BHA21000817-10	PB9916-APC	100 ug	APC	$670		Add to Cart
BHA21000817-11	PB9916-PE	100 ug	PE	$670		Add to Cart
BHA21000817-12	PB9916-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000817-13	PB9916-carrier-free	100 ug	carrier-free	$370		Add to Cart

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