Anti-AKT1 Antibody

Mfr.No.	Y058027
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic, and nuclear after activation by integrin linked protein kinase 1 (ILK1).
Clonality	polyclonal
Description	The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Host	Rabbit
Immunogen	Synthetic peptide conjugated to KLH
Isotype	IgG
Quantity	100 µl
Reactivity	Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Shipping Condition	Ice Packs

Buffer	PBS, pH 7.4 with 0.02% Sodium Azide
Concentration	100µg/100ul
Description	Specificity: AKT1 antibody detects endogenous levels of AKT1 protein. Not tested with other proteins Relevance: The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).
Format	Liquid
Purity	Protein A purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition	-20C

Applications

WB, IF, ELISA

Description

Recommended dilutions Peptide ELISA: 1:32,000 WB: 1/500 - 1/1000 IF:1:50-100. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user, WB ; IF ; ELISA

Dilution

ELISA	1:32,000
WB	1:500-1:1000
IF	1:50-100

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SKU	Supplier No.	Size	Your price	Quantity
BHA10900321	Y058027	100 ul	$217.50		Add to Cart

Anti-AKT1 Antibody

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