This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IHC, ELISA
Description
For research use onlyPeptide ELISA titer: 1:10,000-1:40,000Western blot analysis: 1:1,000-1:2,000Immunohistochemistry: 1:100-1:500Optimal dilutions/concentrations should be determined by the end use., WB ; IHC ; ELISA
Dilution
ELISA
1:10,000-1:40,000
WB
1:1,000-1:2,000
IHC
1:100-1:500
Reference
Chen, M et al. "Targeting primitive chronic myeloid leukemia cells by effective inhibition of a new AHI-1-BCR-ABL-JAK2 complex." J. Natl. Cancer Inst. 105:405-23 (2013). PubMed: 23446755.
Liu, X et al. "Molecular and structural characterization of the SH3 domain of AHI-1 in regulation of cellular resistance of BCR-ABL(+) chronic myeloid leukemia cells to tyrosine kinase inhibitors." Proteomics 12:2094-106 (2012). PubMed: 22623184.