Angiogenin (ANG1), also known as Ribonuclease 5, is a small 123 amino acid protein that in humans is encoded by the ANG gene. The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alteative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
A human recombinant protein corresponding to amino acids Q25-P147 was used as the immunogen for the Angiogenin antibody.
Isotype
IgG
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Uniprot
P03950
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the Angiogenin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, ELISA
Dilution
Weste blot: 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml (human protein tested); request BSA-free format for coating