This recombinant Alpha Tubulin antibody reacts to a-Tubulin, including Tubulin alpha-1A chain and Tubulin alpha-1B chain.
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Immunogen Region
A peptide corresponding to the C-terminus of a-Tubulin was used as the immunogen for this recombinant Alpha Tubulin antibody.
Isotype
Rabbit IgG
Species Reactivity
All Species
Note
The stated application concentrations are suggested starting points. Titration of the recombinant Alpha Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Uniprot
P68363
Format
Purified
Purity
Protein A purified from animal origin-free supeatant
Storage
Store the recombinant Alpha Tubulin antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Applications
WB, IP, ICC, IHC, ChIP
Description
Weste blot: 1:1000,Immunoprecipitation: 1:200,Chromatin IP (ChIP): 1:200,Immunocytochemistry: 1:200,Immunohistochemistry: 1:200 (1)