Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin(alpha A) shares structural homology with spectrin and dystrophin.
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
Rabbit skeletal alpha-actinin was used as the immunogen for this alpha-Actinin antibody.
Isotype
IgG1
Predicted Reactivity
Human, mouse, rat, rabbit
Reactivity
Human, Mouse, Rat, Rabbit
Recombinant
No
Gene Id
88
Clone No
EA-53
Format
Ascites
Purification
Ascites
Storage
After reconstitution, the alpha-Actinin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, IHC-F
Dilution
Western blot: 3-5ug/ml,IHC (Paraffin): 4-7ug/ml (1),Immunohistochemistry (Frozen): 4-7ug/ml to detect in formalin or acetone fixed tissues