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Home / Primary Antibodies

Alpha Actin Antibody / ACTA2 (smooth muscle) (V9076IHC)

SKU : BHA17127911

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NSJ Bioreagents

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Mfr.No.	V9076IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	Actin is a major component of the cytoskeleton and is present in most cell types. This mAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	A 10 amino acid peptide from the alpha smooth muscle isoform of actin was used as the immunogen for this Smooth Muscle Actin antibody.
Isotype	Mouse IgG2a, kappa
Species Reactivity	Human
Note	Optimal dilution of the antibody should be determined by the researcher.

Uniprot	P62736

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Smooth Muscle Actin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	<a href="https://www.nsjbio.com/tds-pdf/smooth-muscle-actin-antibody-spm332-v9076" target="_blank">Datasheet</a>

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SKU	Supplier No.	Size	Your price	Quantity
BHA17127911	V9076IHC-7ML	7 ml	$499		Add to Cart

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