Rabbit Anti-Human AKT1 (pThr308) Polyclonal

Mfr.No.	SPC-1341, SPC-1341D, SPC-1341D-A390, SPC-1341D-A488, SPC-1341D-A594, SPC-1341D-BI, SPC-1341D-FITC
Immunogen Species	Human
Alternative Names	RAC-PK-alpha Antibody, Protein kinase B alpha Antibody, RAC Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, RAC Alpha Antibody, AKT Antibody, EC:2.7.11.1 Antibody, AKT 1 Antibody, PRKBA Antibody, AKT1_HUMAN Antibody, AKT1 Antibody, PKB Antibody, PKB-ALPHA Antibody, MGC99656 Antibody, PKB alpha Antibody, RAC-alpha serine/threonine-protein kinase Antibody
Antigen	AKT1
Categories	Antibodies
Cellular Localization	Cytoskeleton, Nucleus, Plasma Membrane, Cell Membrane, Cytoplasm, Ciliary Basal Body, Microtubule, Spindle, Cytosol, Mitochondrion, Nucleoplasm, Cell-Cell Junction, Postsynapse, Protein Complex, Vesicle
Certificate Of Analysis	A 1:500 dilution of SPC-1341 was sufficient for detection of AKT1 (pThr308) in 10 ug of human embryonic kidney epithelial cell line (hek293) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
Clonality	Polyclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC
Description	Rabbit Anti-Human AKT1 (pThr308) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthetic phospho-peptide from human AKT1 around the phosphorylation site of threonine 308 (pThr308)., Homo Sapiens (Human)
Immunogen Region	Synthetic phospho-peptide from human AKT1 around the phosphorylation site of threonine 308 (pThr308).
Isotype	IgG
Launch Date	2018/03/07
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human
Tissue Specificity	Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.

Swissprot	P31749
Accession Number	NP_001014431.1
Gene Id	207
Target	AKT1 (pThr308)
Research Area	Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases, PKB / AKT, Cancer, Apoptosis, Cancer Metabolism, Cell Cycle, PI3k/AKT Pathway

Buffer	PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
Dilutions	WB (1:250-1:500); IHC (1:25-1:50); ICC/IF (1:50-100); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Purity	Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects endogenous levels of AKT1 only when phosphorylated at threonine 308 (pThr308).
Storage	-20ºC
Storage Buffer	PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB, ICC/IF
Description	WB ; IHC ; ICC ; IF
Dilution	WB (1:250-1:500); IHC (1:25-1:50); ICC/IF (1:50-100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11918324-1	SPC-1341D	Unconjugated	100 ul	$331	Add to Cart
BHA11918324-2	SPC-1341D-A390	ATTO 390	100 ul	$385	Add to Cart
BHA11918324-3	SPC-1341D-A488	ATTO 488	100 ul	$384	Add to Cart
BHA11918324-4	SPC-1341D-A594	ATTO 594	100 ul	$384	Add to Cart
BHA11918324-5	SPC-1341D-BI	Biotin	100 ul	$380	Add to Cart
BHA11918324-6	SPC-1341D-FITC	FITC	100 ul	$375	Add to Cart

AKT1 Antibody (pThr308) - SPC-1341

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