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Home / Primary Antibodies / Monoclonal Antibodies

AKT Antibody / PH domain

SKU : BHA17103816

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NSJ Bioreagents

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Mfr.No.	R20335
Clonality	Monoclonal
Description	Akt isoforms are overexpressed in a variety of human tumors, and, at the genomic level, are amplified in gastric adenocarcinomas (Akt1), ovarian (Akt2), pancreatic (Akt2) and breast (Akt2) cancer. The role of Akt3 is less clear, though it appears to be predominantly expressed in the brain.<br><br>Akt possesses a protein domain known as a PH domain, or Pleckstrin Homology domain, named after Pleckstrin, the protein in which it was first discovered. This domain binds to phosphoinositides with high affinity. In the case of the PH domain of Akt, it binds either PIP3 (phosphatidylinositol (3,4,5)-trisphosphate, PtdIns(3,4,5)P3) or PIP2 (phosphatidylinositol (3,4)-bisphosphate, PtdIns(3,4)P2).(9) This is useful for control of cellular signaling because the di-phosphorylated phosphoinositide PIP2 is only phosphorylated by the family of enzymes, PI 3-kinases (phosphoinositide 3-kinase or PI3-K), and only upon receipt of chemical messengers which tell the cell to begin the growth process. For example, PI 3-kinases may be activated by a G protein coupled receptor or receptor tyrosine kinase such as the insulin receptor. Once activated, PI 3-kinase phosphorylates PIP2 to form PIP3. (Wiki)
Formulation	Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host	Rabbit
Immunogen Region	A peptide corresponding to the PH domain of human AKT1/2/3 was used as the immunogen for the recombinant AKT antibody.
Isotype	IgG
Predicted Reactivity	Human
Reactivity	Human
Recombinant	Yes
Subcellular Location	Cytoplasmic, membranous, nuclear

Uniprot	P31749, P31751, Q9Y243
Clone No	RM316

Format	Purified
Purification	Protein A purified from animal origin-free supernatant
Storage	Store the recombinant AKT antibody at -20°C.

Applications	WB, IHC-P
Dilution	Immunohistochemistry (FFPE): 1:200-1:500,Western Blot: 1:5000-1:10000

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17103816	R20335	100ul	$399		Add to Cart

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