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Home / Antibodies

AKT Antibody / PH domain (R20335)

SKU : BHA17120143

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NSJ Bioreagents

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Mfr.No.	R20335-0.1ML
Clonality	Recombinant Rabbit Monoclonal
Description	Akt isoforms are overexpressed in a variety of human tumors, and, at the genomic level, are amplified in gastric adenocarcinomas (Akt1), ovarian (Akt2), pancreatic (Akt2) and breast (Akt2) cancer. The role of Akt3 is less clear, though it appears to be predominantly expressed in the brain.Akt possesses a protein domain known as a PH domain, or Pleckstrin Homology domain, named after Pleckstrin, the protein in which it was first discovered. This domain binds to phosphoinositides with high affinity. In the case of the PH domain of Akt, it binds either PIP3 (phosphatidylinositol (3,4,5)-trisphosphate, PtdIns(3,4,5)P3) or PIP2 (phosphatidylinositol (3,4)-bisphosphate, PtdIns(3,4)P2).(9) This is useful for control of cellular signaling because the di-phosphorylated phosphoinositide PIP2 is only phosphorylated by the family of enzymes, PI 3-kinases (phosphoinositide 3-kinase or PI3-K), and only upon receipt of chemical messengers which tell the cell to begin the growth process. For example, PI 3-kinases may be activated by a G protein coupled receptor or receptor tyrosine kinase such as the insulin receptor. Once activated, PI 3-kinase phosphorylates PIP2 to form PIP3. (Wiki)
Formulation	Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host	Rabbit
Immunogen Region	A peptide corresponding to the PH domain of human AKT1/2/3 was used as the immunogen for the recombinant AKT antibody.
Isotype	Rabbit IgG
Species Reactivity	Human
Note	The stated application concentrations are suggested starting points. Titration of the recombinant AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Uniprot	P31749, P31751, Q9Y243

Format	Purified
Purity	Protein A purified from animal origin-free supernatant
Storage	Store the recombinant AKT antibody at -20oC.

Applications	WB, IHC-P
Description	Immunohistochemistry (FFPE): 1:200-1:500,Western Blot: 1:5000-1:10000

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17120143	R20335-0.1ML	100ul	$409		Add to Cart

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