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Home / Primary Antibodies

Actin Antibody (pan muscle) (V2318SAF)

SKU : BHA17123668

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NSJ Bioreagents

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Mfr.No.	V2318SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	SDS extract of human myocardium was used as the immunogen for this Muscle Actin HHF35 antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human, Mouse
Note	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P62736, P68032, P68133

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Muscle Actin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, IHC-P
Description	Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123668	V2318SAF-100UG	100 ug	$499		Add to Cart

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