ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A recombinant protein fragment from the N-terminal region of human ACTH was used as the immunogen for the recombinant ACTH antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human, Mouse and Rat (broad species reactivity predicted)
Note
Optimal dilution of the recombinant ACTH antibody should be determined by the researcher.
Uniprot
P01189
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the recombinant ACTH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Applications
ELISA, IHC-P
Description
ELISA (For coating order BSA-free format),Immunohistochemistry (FFPE): 1-2ug/ml