This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A mixture of erythrocytes of blood group A and glycoprotein fraction isolated from the saliva of secretors with blood group A was used as the immunogen for the ABO antibody.
Isotype
Mouse IgM, kappa
Species Reactivity
Human
Note
Optimal dilution of the ABO antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Uniprot
P16442
Format
Purified
Purity
PEG precipitation
Storage
Store the ABO antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IH, IF
Description
Immunofluorescence: 2-4ug/ml,Immunohistology (formalin-fixed): 1-2ug/ml for 30 min at RT