This recombinant 5mC antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
Formulation
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Immunogen Region
BSA-conjugated 5-methylcytosine was used as the immunogen for this recombinant 5mC antibody.
Isotype
Rabbit IgG
Species Reactivity
Human
Note
The stated application concentrations are suggested starting points. Titration of the recombinant 5mC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Format
Purified
Purity
Protein A purified from animal origin-free supeatant
Storage
Store the recombinant 5mC antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).