PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.
Product Type
Recombinant Proteins
Tag
GST
Field of Use
Enzymes
Molecular Weight
46 kDa
Application
Recombinant protein tag removal, on-site cleavage, recombinant protein characterization, ELISA, Western blot, crystallization studies.
Expression Source
E. coli
Uniprot
NP_740524.1 (Genbank)
Shipping
Dry Ice
Purity
>90%, SDS-PAGE
Storage
Stable for 12 months at -80°C, Avoid freeze/thaw cycles