Rabbit Anti-Human TLR4 Polyclonal

Mfr.No.	SPC-200, SPC-200D, SPC-200D-A390, SPC-200D-A488, SPC-200D-A594, SPC-200D-ALP, SPC-200D-APC, SPC-200D-BI, SPC-200D-FITC, SPC-200D-HRP, SPC-200D-PCP, SPC-200D-RPE, SPC-200S
Immunogen Species	Human
Alternative Names	ARMD10 Antibody, CD284 Antibody, TLR 4 Antibody, TOLL Antibody, Toll like receptor 4 Antibody
Categories	Antibodies
Cellular Localization	Membrane
Certificate Of Analysis	2 ug/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human TLR4 Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4, Homo Sapiens (Human)
Immunogen Region	Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
Launch Date	2011/01/01
Net Weight (g)	0.1
Reactivity	Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Human

Swissprot	O00206
Accession Number	NP_612564.1
Gene Id	7099
Target	TLR4
Research Area	Immunology, Innate Immunity, Immunoglobulins, Immunoglobulin Receptors, Cardiovascular System, Atherosclerosis, Vascular Inflammation, Inflammatory Mediators, Adaptive Immunity, Regulatory T Cells, TLR Signaling, Macrophage

Buffer	PBS, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:500), IHC (1:50); optimal dilutions for assays should be determined by the user.
Purification	Protein A Purified
Scientific Background	The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	FACS, IHC, WB, ICC/IF, FCM
Description	WB ; IHC ; ICC ; IF ; FCM ; FACS
Dilution	WB (1:500), IHC (1:50); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Muzio M., Natoli G., Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101. 2. Medzhitov R. and Janeway C.A (1997) Cell. 91: 295-298. 3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta. 1518(1-2): 157-161. 4. Takeuchi O., et al. (1999) Immunity. 11: 443. 5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA. 97: 2163-2167. 6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature. 388(6640): 394-397.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11907376-1	SPC-200D	Unconjugated	100 ug	$295	Add to Cart
BHA11907376-2	SPC-200D-A390	ATTO 390	100 ug	$349	Add to Cart
BHA11907376-3	SPC-200D-A488	ATTO 488	100 ug	$348	Add to Cart
BHA11907376-4	SPC-200D-A594	ATTO 594	100 ug	$348	Add to Cart
BHA11907376-5	SPC-200D-ALP	Alkaline Phosphatase	100 ug	$341	Add to Cart
BHA11907376-6	SPC-200D-APC	APC	100 ug	$347	Add to Cart
BHA11907376-7	SPC-200D-BI	Biotin	100 ug	$343	Add to Cart
BHA11907376-8	SPC-200D-FITC	FITC	100 ug	$339	Add to Cart
BHA11907376-9	SPC-200D-HRP	HRP	100 ug	$334	Add to Cart
BHA11907376-10	SPC-200D-PCP	PerCP	100 ug	$347	Add to Cart
BHA11907376-11	SPC-200D-RPE	RPE	100 ug	$344	Add to Cart
BHA11907376-12	SPC-200S	Unconjugated	12 ug	$44	Add to Cart

TLR4 Antibody - SPC-200