Rabbit Anti-Human RAS Polyclonal

Mfr.No.	SPC-173, SPC-173D, SPC-173C, SPC-173D-A390, SPC-173D-A488, SPC-173D-A594, SPC-173D-ALP, SPC-173D-APC, SPC-173D-BI, SPC-173D-FITC, SPC-173D-HRP, SPC-173D-PCP, SPC-173D-RPE, SPC-173S
Immunogen Species	Human
Alternative Names	GTPase Hras Antibody, GTPase Kras Antibody, GTPase Nras Antibody, Ha Ras Antibody, K Ras Antibody, K ras p21 Antibody, KRAS Antibody, NRAS Antibody, p21ras Antibody, RASH2 Antibody
Categories	Antibodies
Cellular Localization	Golgi Apparatus Membrane, Cell Membrane, Golgi Apparatus
Certificate Of Analysis	1 ug/ml of SPC-173 was sufficient for detection of Ras in 20 ug of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human RAS Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over th, Homo Sapiens (Human), Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over th
Immunogen Region	Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
Launch Date	2012/07/26
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human, Bovine
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Bovine, Human

Swissprot	B2KGV5
Accession Number	NP_035067.2
Gene Id	18176
Target	RAS
Research Area	Cancer, Cell Signaling, Epigenetics and Nuclear Signaling

Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), IHC (1:100), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~21kDa.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB, ICC/IF
Description	WB ; IHC ; ICC ; IF
Dilution	WB (1:1000), IHC (1:100), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Boguski M.S. and McCormick F. (1993) Nature 366: 643654. 2. Avruch J., et al. (1994) Trends Biochem. Sci.19: 279283. 3. Buday L. and Downward J. (1993) Cell 73: 611620. 4. Huang D.C., et al. (1993) Mol. Cell Biol. 13: 24202431. 5. Bos J.L. (1989) Cancer Res. 49: 46824689. 6. Ma J. and Karplus M. (1997) J. Mol. Biol. 274:114131.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11907021-1	SPC-173D	Unconjugated	100 ug	$283	Add to Cart
BHA11907021-2	SPC-173C	Unconjugated	25 ug	$125	Add to Cart
BHA11907021-3	SPC-173D-A390	ATTO 390	100 ug	$337	Add to Cart
BHA11907021-4	SPC-173D-A488	ATTO 488	100 ug	$336	Add to Cart
BHA11907021-5	SPC-173D-A594	ATTO 594	100 ug	$336	Add to Cart
BHA11907021-6	SPC-173D-ALP	Alkaline Phosphatase	100 ug	$329	Add to Cart
BHA11907021-7	SPC-173D-APC	APC	100 ug	$334	Add to Cart
BHA11907021-8	SPC-173D-BI	Biotin	100 ug	$331	Add to Cart
BHA11907021-9	SPC-173D-FITC	FITC	100 ug	$327	Add to Cart
BHA11907021-10	SPC-173D-HRP	HRP	100 ug	$322	Add to Cart
BHA11907021-11	SPC-173D-PCP	PerCP	100 ug	$334	Add to Cart
BHA11907021-12	SPC-173D-RPE	RPE	100 ug	$332	Add to Cart
BHA11907021-13	SPC-173S	Unconjugated	12 ug	$44	Add to Cart

RAS Antibody - SPC-173

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