Rabbit Anti-Human PUMA (CT) Polyclonal

Mfr.No.	SPC-166, SPC-166D, SPC-166C, SPC-166D-A390, SPC-166D-A488, SPC-166D-A594, SPC-166D-ALP, SPC-166D-APC, SPC-166D-BI, SPC-166D-FITC, SPC-166D-HRP, SPC-166D-PCP, SPC-166D-RPE, SPC-166S
Immunogen Species	Human
Alternative Names	BBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody
Categories	Antibodies
Cellular Localization	Mitochondrion
Certificate Of Analysis	1 ug/ml of SPC-166 was sufficient for detection of PUMA in 20 ug of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human PUMA (CT) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	C-terminal amino acids of human PUMA, Homo Sapiens (Human)
Immunogen Region	C-terminal amino acids of human PUMA
Launch Date	2007/06/01
Net Weight (g)	0.1
Reactivity	Mouse, Human, Chicken
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Chicken, Human

Swissprot	Q9BXH1
Accession Number	NP_001120712.1
Gene Id	27113
Target	PUMA
Research Area	Cancer, Apoptosis, Intracellular, Bcl2 Family, Invasion/microenvironment, Cell Signaling, Metabolism, Mitochondrial Metabolism

Buffer	PBS, 0.02% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Protein A Purified
Scientific Background	Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAbeta.
Storage	-20ºC
Storage Buffer	PBS, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94. 2. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82. 3. Han J., et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11906903-1	SPC-166D	Unconjugated	100 ug	$330	Add to Cart
BHA11906903-2	SPC-166C	Unconjugated	25 ug	$156	Add to Cart
BHA11906903-3	SPC-166D-A390	ATTO 390	100 ug	$384	Add to Cart
BHA11906903-4	SPC-166D-A488	ATTO 488	100 ug	$383	Add to Cart
BHA11906903-5	SPC-166D-A594	ATTO 594	100 ug	$383	Add to Cart
BHA11906903-6	SPC-166D-ALP	Alkaline Phosphatase	100 ug	$376	Add to Cart
BHA11906903-7	SPC-166D-APC	APC	100 ug	$382	Add to Cart
BHA11906903-8	SPC-166D-BI	Biotin	100 ug	$378	Add to Cart
BHA11906903-9	SPC-166D-FITC	FITC	100 ug	$374	Add to Cart
BHA11906903-10	SPC-166D-HRP	HRP	100 ug	$370	Add to Cart
BHA11906903-11	SPC-166D-PCP	PerCP	100 ug	$382	Add to Cart
BHA11906903-12	SPC-166D-RPE	RPE	100 ug	$380	Add to Cart
BHA11906903-13	SPC-166S	Unconjugated	12 ug	$44	Add to Cart

PUMA (CT) Antibody - SPC-166