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Home / Primary Antibodies / Polyclonal Antibodies

PKC alpha Antibody - SPC-1191

BHA11916236
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+1 866.986.9598
Email
[email protected]
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Swissprot P17252
Accession Number NP_002728
Gene Id 5578
Target PKC alpha
Research Area Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases, PKC, Cardiovascular System, Heart, Hypertrophy
Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Dilutions WB (1:250); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Purity Highly expressed in the brain, blood, blood vessels, and prostate.
Scientific Background Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumourigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. Is highly expressed in a number of cancer cells where it can act as a tumour promoter and is implicated in malignant phenotypes of several tumours such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity.
Shipping Temperature Blue Ice or 4°C
Specificity Detects 76.764 kDa.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications AM, WB
Description WB ; AM
Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11916236-1 SPC-1191D Unconjugated 100 ul $331
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BHA11916236-2 SPC-1191D-A390 ATTO 390 100 ul $385
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BHA11916236-3 SPC-1191D-A488 ATTO 488 100 ul $384
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BHA11916236-4 SPC-1191D-A594 ATTO 594 100 ul $384
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BHA11916236-5 SPC-1191D-BI Biotin 100 ul $380
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BHA11916236-6 SPC-1191D-FITC FITC 100 ul $375
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