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Home / Primary Antibodies / Polyclonal Antibodies

Phosphotyrosine Antibody - SPC-161

BHA11906847
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+1 866.986.9598
Email
[email protected]
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Target Phosphotyrosine
Research Area Cell Signaling, Post-translational Modifications, Phosphorylation
Buffer PBS pH7.0, 50% glycerol, 0.01% sodium azide
Concentration 0.25 mg/ml
Dilutions WB (1:500), IHC (1:100), ICC/IF (1:50), ELISA (1:2000), IP (1:100); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Scientific Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are
helpful in facilitating the identification of tyrosine kinase substrates (6).
Shipping Temperature Blue Ice or 4°C
Specificity Detects proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.
Storage -20ºC
Storage Buffer PBS pH7.0, 50% glycerol, 0.01% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP ; ELISA
Dilution WB (1:500), IHC (1:100), ICC/IF (1:50), ELISA (1:2000), IP (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187.

2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365.

3. Downward J. (2001) Nature 411: 759-762.

4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678.

5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352.

6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656.

7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906847-1 SPC-161F Unconjugated 400 ul $341
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BHA11906847-2 SPC-161F-A390 ATTO 390 400 ul $395
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BHA11906847-3 SPC-161F-A488 ATTO 488 400 ul $394
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BHA11906847-4 SPC-161F-A594 ATTO 594 400 ul $394
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BHA11906847-5 SPC-163F Biotin 400 ul $404
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BHA11906847-6 SPC-161F-FITC FITC 400 ul $385
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