Rabbit Anti-Rat NKCC2 Polyclonal

Mfr.No.	SPC-401, SPC-401D, SPC-401D-A390, SPC-401D-A488, SPC-401D-A594, SPC-401D-ALP, SPC-401D-APC, SPC-401D-BI, SPC-401D-FITC, SPC-401D-HRP, SPC-401D-PCP, SPC-401D-RPE, SPC-401S
Immunogen Species	Rat
Alternative Names	SLC12A1 Antibody, sodium potassium chloride cotransporter 2 Antibody, sodium-potassium-chloride cotransporter isoform 2 Antibody, BSC1 Antibody, MBSC1 Antibody, Na-K-2Cl cotransporter Antibody, NKCC2A variant A Antibody, RBSC Antibody
Categories	Antibodies
Cellular Localization	Membrane
Certificate Of Analysis	1 ug/ml of SPC-401 was sufficient for detection of NKCC2 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Rat NKCC2 Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224)., Rattus Norvegicus (Rat)
Immunogen Region	Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).
Launch Date	2010/06/11
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Rattus Norvegicus (Rat)
Species Reactivity Medium	Mouse, Rat, Human

Swissprot	Q5PQV1
Accession Number	NP_001257546.1
Gene Id	25065
Target	NKCC2
Research Area	Cancer, Pumps/Transporters, Ion Pumps/Transporters, Neuroscience

Buffer	PBS, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein A Purified
Scientific Background	NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~160kDa.
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB
Description	WB ; IHC
Dilution	WB (1:1000); IHC (1:25); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Caceres P.S., Ares G.R., and Ortiz P.A. (2009) J Biol Chem. 284(37): 24965-24971. 2. Xue H., et al. (2009) Neurogastroentrol Motil. 21(10): 1068-1089.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11907767-1	SPC-401D	Unconjugated	100 ug	$341	Add to Cart
BHA11907767-2	SPC-401D-A390	ATTO 390	100 ug	$395	Add to Cart
BHA11907767-3	SPC-401D-A488	ATTO 488	100 ug	$394	Add to Cart
BHA11907767-4	SPC-401D-A594	ATTO 594	100 ug	$394	Add to Cart
BHA11907767-5	SPC-401D-ALP	Alkaline Phosphatase	100 ug	$387	Add to Cart
BHA11907767-6	SPC-401D-APC	APC	100 ug	$393	Add to Cart
BHA11907767-7	SPC-401D-BI	Biotin	100 ug	$389	Add to Cart
BHA11907767-8	SPC-401D-FITC	FITC	100 ug	$385	Add to Cart
BHA11907767-9	SPC-401D-HRP	HRP	100 ug	$381	Add to Cart
BHA11907767-10	SPC-401D-PCP	PerCP	100 ug	$393	Add to Cart
BHA11907767-11	SPC-401D-RPE	RPE	100 ug	$391	Add to Cart
BHA11907767-12	SPC-401S	Unconjugated	12 ug	$44	Add to Cart

NKCC2 Antibody - SPC-401