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Home / Primary Antibodies / Polyclonal Antibodies

KDEL Antibody - SPC-109

SKU : BHA11906384

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StressMarq Biosciences Inc.

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Mfr.No.	SPC-109, SPC-109B, SPC-109A, SPC-109B-A390, SPC-109B-A488, SPC-109B-A594, SPC-109B-ALP, SPC-109B-APC, SPC-109B-BI, SPC-109B-FITC, SPC-109B-HRP, SPC-109B-PCP, SPC-109B-RPE, SPC-109S
Alternative Names	Lys Asp Glu Leu Antibody
Categories	Antibodies
Cellular Localization	Endoplasmic Reticulum
Certificate Of Analysis	A 1:1000 dilution of SPC-109 was sufficient for detection of KDEL-containing proteins in 20 ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.
Clonality	Polyclonal
Description	Rabbit Anti-KDEL Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	KDEL containing peptide immunogen
Immunogen Region	KDEL containing peptide immunogen
Launch Date	2011/05/16
Net Weight (g)	0.2
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species Reactivity Medium	Mouse, Rat, Human

Target	KDEL
Research Area	Cell Signaling, Tags and Cell Markers, Organelle Markers, Chaperone Proteins, Protein Trafficking

Buffer	PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Protein A Purified
Scientific Background	The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.
Storage	-20ºC
Storage Buffer	PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB, ICC/IF
Description	WB ; ICC ; IF ; IHC
Dilution	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Ozawa K., et al. (2008) Mol Pharmacol. 74:1610. 2. Austin R.C., et al. (2003) J Biol Chem. 278: 17438.

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Combination SKU	Supplier No.	Conjugate	Size	Your price	Quantity
BHA11906384-1	SPC-109B	Unconjugated	200 ug	$327		Add to Cart
BHA11906384-2	SPC-109A	Unconjugated	50 ug	$161		Add to Cart
BHA11906384-3	SPC-109B-A390	ATTO 390	200 ug	$429		Add to Cart
BHA11906384-4	SPC-109B-A488	ATTO 488	200 ug	$426		Add to Cart
BHA11906384-5	SPC-109B-A594	ATTO 594	200 ug	$426		Add to Cart
BHA11906384-6	SPC-109B-ALP	Alkaline Phosphatase	200 ug	$413		Add to Cart
BHA11906384-7	SPC-109B-APC	APC	200 ug	$425		Add to Cart
BHA11906384-8	SPC-109B-BI	Biotin	200 ug	$415		Add to Cart
BHA11906384-9	SPC-109B-FITC	FITC	200 ug	$407		Add to Cart
BHA11906384-10	SPC-109B-HRP	HRP	200 ug	$400		Add to Cart
BHA11906384-11	SPC-109B-PCP	PerCP	200 ug	$425		Add to Cart
BHA11906384-12	SPC-109B-RPE	RPE	200 ug	$417		Add to Cart
BHA11906384-13	SPC-109S	Unconjugated	12 ug	$44		Add to Cart

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