Rabbit Anti-Human HSP40 Polyclonal

Mfr.No.	SPC-100, SPC-100D, SPC-100D-A390, SPC-100D-A488, SPC-100D-A594, SPC-100D-ALP, SPC-100D-APC, SPC-100D-BI, SPC-100D-FITC, SPC-100D-HRP, SPC-100D-PCP, SPC-100D-RPE, SPC-100S
Immunogen Species	Human
Alternative Names	DnaJ (HSP40) homolog subfamily B member 1 Antibody, DNAJ1 Antibody, DNAJB1 Antibody, HDJ1 Antibody, HSPF1 Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm, Nucleus
Certificate Of Analysis	0.5 ug/ml of SPC-100 was sufficient for detection of HSP40 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human HSP40 Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Recombinant purified full-length human HSP40 (no tags), Homo Sapiens (Human)
Immunogen Region	Recombinant purified full-length human HSP40 (no tags)
Launch Date	2007/06/01
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human

Swissprot	P25685
Accession Number	NP_006136.1
Gene Id	3337
Target	HSP40
Research Area	Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Protein Trafficking

Buffer	Rabbit Antiserum
Concentration	N/A
Dilutions	WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Rabbit Antiserum
Scientific Background	DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s. HSP40, also known as HDJ1 (6), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-5). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (2).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~40kDa.
Storage	-20ºC
Storage Buffer	Rabbit Antiserum *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IP, WB, ICC/IF
Description	WB ; ICC ; IF ; IP
Dilution	WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Melville, M. W. et al. (1997) PNAS USA, 94: 97-102. 2. Hattori, H., Liu, Y-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K., and Ohtsuka, K. (1992) Cell Structure and Function 17: 77-86. 3. Ohtsuka, K. Masuda, A., Nakai, A., and Nagata, K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647. 4. Bardwell, J.C.A., Tilly, K., Craig, E., King, J., Zylicz, M. and Georgopoulos, C. (1986) J. Biol. Chem. 261: 1782-1785. 5. Ohku, M., Tamura, F., Nishimura, S., and Uchida, H. (1986) J. Biol. Chem. 261: 1778-1781. 6. Ohtsuka, K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11906205-1	SPC-100D	Unconjugated	100 ul	$327	Add to Cart
BHA11906205-2	SPC-100D-A390	ATTO 390	100 ul	$381	Add to Cart
BHA11906205-3	SPC-100D-A488	ATTO 488	100 ul	$380	Add to Cart
BHA11906205-4	SPC-100D-A594	ATTO 594	100 ul	$380	Add to Cart
BHA11906205-5	SPC-100D-ALP	Alkaline Phosphatase	100 ul	$373	Add to Cart
BHA11906205-6	SPC-100D-APC	APC	100 ul	$378	Add to Cart
BHA11906205-7	SPC-100D-BI	Biotin	100 ul	$375	Add to Cart
BHA11906205-8	SPC-100D-FITC	FITC	100 ul	$371	Add to Cart
BHA11906205-9	SPC-100D-HRP	HRP	100 ul	$366	Add to Cart
BHA11906205-10	SPC-100D-PCP	PerCP	100 ul	$378	Add to Cart
BHA11906205-11	SPC-100D-RPE	RPE	100 ul	$376	Add to Cart
BHA11906205-12	SPC-100S	Unconjugated	12 ul	$44	Add to Cart

HSP40 Antibody - SPC-100