Rabbit Anti-Plant HSP101/ClpB CT (Maize) Polyclonal

Mfr.No.	SPC-306, SPC-306B
Immunogen Species	Plant
Alternative Names	heat shock protein 1010 Antibody, HOT1 Antibody, HSP101 Antibody, HSPS Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	0.7 ul/ml of SPC-306 was sufficient for detection of HSP101 in 30 ug of mature embryo maize extracts by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Plant HSP101/ClpB CT (Maize) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	15aa peptide sequence from the C-terminus of maize HSP101. Gene accession number AF077337.
Immunogen Region	15aa peptide sequence from the C-terminus of maize HSP101. Gene accession number AF077337.
Launch Date	2009/01/01
Net Weight (g)	0.2
Reactivity	Maize, Plant
Recombinant	FALSE
Regulatory	RUO
Species	Plant
Species Reactivity Medium	Plant

Swissprot	Q9S822
Accession Number	NP_001104935.1
Gene Id	541780
Target	HSP101
Research Area	Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Protein Trafficking

Buffer	Lyophilized rabbit Antiserum. For reconstitution add 200 µl of sterile water.
Dilutions	WB (1:2000); optimal dilutions for assays should be determined by the user.
Purification	Rabbit Antiserum
Scientific Background	HSPs/chaperones are known to be expressed in plants not only when they experience high temperatures stress, but also in response to a wide range of other environmental stresses such as water stress, salt stress, cold stress and oxidative stress (2, 3). HSP100 is one such major class that acts as a chaperone. HSP101/ClpB is a member of this HSP100 family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones. In spite of expression during heat stress, members of HSP100 protein family are also expressed during seed development. HSP101 protein is both nuclear and cytoplasmic-localized (1, 4).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects the C-terminal domain of HSP101 ~101kDa. No reactivity to other cereal HSP101.
Storage	-20ºC
Storage Buffer	Lyophilized rabbit Antiserum. For reconstitution add 200 ul of sterile water.
Storage Temperature	-20°C

Applications	WB
Description	WB
Dilution	WB (1:2000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Nieto-Sotelo J., et al. (2002) Plant Cell. 14: 1621-1633. 2. Vierling E. (1991) Annu Rev. Plabt Physiol. Plant Mol. Biol. 42: 579-620. 3. Waters W., et al. (2004) Trends Plant Sci. 9: 244-252. 4. Nieto-Sotelo J., Kannan K.B., Martinex L.M. and Segal C. (1999) Gene. 230: 187-195.

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Combination SKU	Supplier No.	Conjugate	Size	Your price	Quantity
BHA11907717-1	SPC-306B	Unconjugated	200 ul	$345		Add to Cart

HSP101/ClpB CT (Maize) Antibody - SPC-306