Rabbit Anti-Human Caspase 3 (cleaved) Polyclonal

Mfr.No.	SPC-1319, SPC-1319D, SPC-1319D-A390, SPC-1319D-A488, SPC-1319D-A594, SPC-1319D-BI, SPC-1319D-FITC
Immunogen Species	Human
Alternative Names	Apopain antibody, CASP-3 antibody, CASP3 antibody, CASP3_HUMAN antibody, Caspase 3 antibody, caspase 3, apoptosis-related cysteine protease antibody, Caspase-3 subunit p12 antibody, CPP 32 antibody, CPP-32 antibody, Cysteine protease CPP32 antibody, PARP cleavage protease antibody, Procaspase 3 antibody, Protein Yama antibody, SCA-1 antibody, SCA1 antibody, SREBP cleavage activity 1 antibody, Yama antibody, Yama protein antibody
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	A 1:1000 dilution of SPC-1319 was sufficient for detection of Caspase 3 in 10 ug of COLO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human Caspase 3 (cleaved) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthesized peptide derived from human Caspase 3., Homo Sapiens (Human)
Immunogen Region	Synthesized peptide derived from human Caspase 3.
Isotype	IgG
Launch Date	2016/09/06
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human

Swissprot	P42574
Accession Number	NP_116786.1
Gene Id	836
Target	Caspase 3 (cleaved)
Research Area	Neurodegeneration, Alzheimer's Disease, Cancer, Apoptosis, Caspases, Cancer Metabolism, Post-translational Modifications, Proteolysis, Neuroscience

Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Dilutions	WB (1:2000), IHC (200); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Caspase-3 is activated in the apoptotic cell both by extrinsic (death ligand) and intrinsic (mitochondrial) pathways. The zymogen feature of caspase-3 is necessary because if unregulated, caspase activity would kill cells indiscriminately. Caspase-3 has been found to be necessary for normal brain development as well as its typical role in apoptosis, where it is responsible for chromatin condensation and DNA fragmentation. Elevated levels of a fragment of Caspase-3, p17, in the bloodstream is a sign of a recent myocardial infarction. It is now being shown that caspase-3 may play a role in embryonic and hematopoietic stem cell differentiation.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB
Description	WB ; IHC
Dilution	WB (1:2000), IHC (200); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Alnemri ES. et al. (1996) Cell 87 (2): 171. 2. Salvesen GS. (2002) Cell Death Differ. 9 (1): 35. 3. Ghavami S (2009) J. Med. Genet. 46 (8): 497510. 4. Porter AG and Jänicke RU (1999) Cell Death Differ. 6 (2): 99104. 5. Agosto M (2011) J. Am. Coll. Cardiol. 57 (2): 2201. 6. Abdul-Ghani M. (2008) Cell Stem Cell 2 (6): 5156.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11917928-1	SPC-1319D	Unconjugated	100 ul	$286	Add to Cart
BHA11917928-2	SPC-1319D-A390	ATTO 390	100 ul	$340	Add to Cart
BHA11917928-3	SPC-1319D-A488	ATTO 488	100 ul	$339	Add to Cart
BHA11917928-4	SPC-1319D-A594	ATTO 594	100 ul	$339	Add to Cart
BHA11917928-5	SPC-1319D-BI	Biotin	100 ul	$334	Add to Cart
BHA11917928-6	SPC-1319D-FITC	FITC	100 ul	$330	Add to Cart

Caspase 3 Antibody (cleaved) - SPC-1319