Rabbit Anti-Human ATG4A Polyclonal

Mfr.No.	SPC-628, SPC-628D, SPC-628D-A390, SPC-628D-A488, SPC-628D-A594, SPC-628D-ALP, SPC-628D-APC, SPC-628D-BI, SPC-628D-FITC, SPC-628D-HRP, SPC-628D-PCP, SPC-628D-RPE, SPC-628S
Immunogen Species	Human
Alternative Names	ATG4 autophagy related 4 homolog A (S. cerevisiae) Antibody, MGC107179 Antibody, AV169859 Antibody, Autophagy-related cysteine endopeptidase 2 Antibody, hAPG4A Antibody, EC:3.4.22.- Antibody, ATG4A Antibody, Autophagin 2 Antibody, Autophagy related protein 4 homolog A Antibody, Cysteine protease ATG4A Antibody, AUTL2 Antibody, Autl2 Antibody, Autophagy-related protein 4 homolog A Antibody, Atg4al Antibody, Autophagin-2 Antibody, Autophagy related 4A cysteine peptidase Antibody, AUT like 2 cysteine endopeptidase Antibody, Autophagy related cysteine endopeptidase 2 Antibody, Apg4a Antibody, AUT-like 2 cysteine endopeptidase Antibody, APG4A Antibody, AI627006 Antibody, ATG4A_HUMAN Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	A 1:1000 dilution of SPC-628 was sufficient for detection of ATG4A in 15 ug of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human ATG4A Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide from the mid-protein of Human ATG4A (aa. 102-113), Homo Sapiens (Human)
Immunogen Region	Synthetic peptide from the mid-protein of Human ATG4A (aa. 102-113)
Launch Date	2016/08/29
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human
Tissue Specificity	Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.

Swissprot	Q8WYN0
Accession Number	NP_001308216.1
Gene Id	115201
Target	ATG4A
Research Area	Cancer, Autophagy, Cardiovascular System, Heart, Cell Signaling

Buffer	PBS, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Purity	Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
Scientific Background	Autophagy related 4A, cysteine peptidase (ATG4A or APG4A) is a C-54 cysteine protease that also belongs to the family of autophagin proteins, and is primarily found in skeletal muscle and brain. ATG4A is involved in cellular autophagy and has been studied as a target for cancer therapy. Specifically it cleaves GATE-16. Mutations in the ATG4a gene is critical for redox regulation and inhibits autophagosome formation.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~45 kDa.
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Mariño G., et al. (2003) J Biol Chem. 278: 3671-8. 2. Scherz-Shouval R., et al. (2003) J Biol Chem. 278: 14053-8. 3. Li M., et al. (2011) J Biol Chem. 286: 7327-38. 4. Scherz-Shouval R., et al. (2007) EMBO J. 26: 1749-60.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11908375-1	SPC-628D	Unconjugated	100 ug	$331	Add to Cart
BHA11908375-2	SPC-628D-A390	ATTO 390	100 ug	$385	Add to Cart
BHA11908375-3	SPC-628D-A488	ATTO 488	100 ug	$384	Add to Cart
BHA11908375-4	SPC-628D-A594	ATTO 594	100 ug	$384	Add to Cart
BHA11908375-5	SPC-628D-ALP	Alkaline Phosphatase	100 ug	$377	Add to Cart
BHA11908375-6	SPC-628D-APC	APC	100 ug	$383	Add to Cart
BHA11908375-7	SPC-628D-BI	Biotin	100 ug	$380	Add to Cart
BHA11908375-8	SPC-628D-FITC	FITC	100 ug	$375	Add to Cart
BHA11908375-9	SPC-628D-HRP	HRP	100 ug	$371	Add to Cart
BHA11908375-10	SPC-628D-PCP	PerCP	100 ug	$383	Add to Cart
BHA11908375-11	SPC-628D-RPE	RPE	100 ug	$381	Add to Cart
BHA11908375-12	SPC-628S	Unconjugated	12 ug	$44	Add to Cart

ATG4A Antibody - SPC-628

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