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Home / Primary Antibodies / Polyclonal Antibodies

Anti-PKC epsilon/PRKCE Antibody Picoband®

SKU : BHA21000185

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Mfr.No.	PB9320, PB9320-10ug, PB9320-Biotin, PB9320-Cy3, PB9320-Dylight488, PB9320-Dylight550, PB9320-Dylight594, PB9320-FITC, PB9320-HRP, PB9320-APC, PB9320-PE, PB9320-iFluor647, PB9320-carrier-free
Alternative Names	Protein kinase C epsilon type
Background	Protein kinase C epsilon type, also known as PKCE, is an enzyme that in humans is encoded by the PRKCE gene. The protein encoded by this gene is one of the PKC family members. PRKCE is mapped to 2p21. This kinase has been shown to be involved in many different cellular functions, such as apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. It has been found that activation of PRKCE can induce VR1 channel activity at room temperature in the absence of any other agonist. PRKCE gene plays a role in apoptosis signaling pathways in thyroid cells and it has been indicated that a naturally occurring PRKCE mutant that functions as a dominant negative can block cell death triggered by a variety of stimuli. Expression of PRKCE inhibits chemotherapy-induced caspase-3 activation and apoptosis, thereby leading to cell survival.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-PKC epsilon/PRKCE Antibody Picoband® catalog # PB9320. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration a
Host	Rabbit
Immunogen Region	E.coli-derived human PKC epsilon recombinant protein (Position: Q53-R236). Human PKC epsilon shares 99% amino acid (aa) sequence identity with both mouse and rat PKC epsilon.
Isotype	Rabbit IgG
Molecular Weight	88 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Protein kinase C epsilon type;2.7.11.13;nPKC-epsilon;PRKCE;PKCE;
Note	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	Q02156
Gene Id	5581
Gene Name	PRKCE
Sequence	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Subcellular Location	Cytoplasm . Cytoplasm, cytoskeleton . Cell membrane . Cytoplasm, perinuclear region . Nucleus . Translocated to plasma membrane in epithelial cells stimulated by HGF. Associated with the Golgi at the perinuclear site in pre-passage fibroblasts (By similarity). In passaging cells, translocated to the cell periphery (By similarity). Translocated to the nucleus in PMA- treated cells (By similarity). .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IHC, WB
Description	Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Mouse, Rat, Human, By Heat Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat

Reference	1. Basta P, Strickland MB, Holmes W, Loomis CR, Ballas LM, Bus DJ (Nov 1992). "Sequence and expression of human protein kinase C-epsilon". Biochim Biophys Acta 1132 (2): 15460. 2. Knauf, J. A., Elisei, R., Mochly-Rosen, D., Liron, T., Chen, X.-N., Gonsky, R., Korenberg, J. R., Fagin, J. A. Involvement of protein kinase C-epsilon (PKC-epsilon) in thyroid cell death: a truncated chimeric PKC-epsilon cloned from a thyroid cancer cell line protects thyroid cells from apoptosis. J. Biol. Chem. 274: 23414-23425, 1999. 3. Premkumar, L. S., Ahe, G. P. Induction of vanilloid receptor channel activity by protein kinase C. Nature 408: 985-990, 2000.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21000185-1	PB9320	100 ug	-	$370		Add to Cart
BHA21000185-2	PB9320-10ug	10 ug	Sample	$99		Add to Cart
BHA21000185-3	PB9320-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21000185-4	PB9320-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21000185-5	PB9320-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21000185-6	PB9320-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21000185-7	PB9320-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21000185-8	PB9320-FITC	100 ug	FITC	$570		Add to Cart
BHA21000185-9	PB9320-HRP	100 ug	HRP	$570		Add to Cart
BHA21000185-10	PB9320-APC	100 ug	APC	$670		Add to Cart
BHA21000185-11	PB9320-PE	100 ug	PE	$670		Add to Cart
BHA21000185-12	PB9320-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21000185-13	PB9320-carrier-free	100 ug	carrier-free	$370		Add to Cart

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