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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Nanog Antibody Picoband®

SKU : BHA21003805

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Mfr.No.	A00153-4, A00153-4-10ug, A00153-4-Biotin, A00153-4-Cy3, A00153-4-Dylight488, A00153-4-Dylight550, A00153-4-Dylight594, A00153-4-FITC, A00153-4-HRP, A00153-4-APC, A00153-4-PE, A00153-4-iFluor647, A00153-4-carrier-free
Alternative Names	Homeobox protein NANOG
Background	NANOG (pron. nanOg) is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. In humans, this protein is encoded by the NANOG gene. It is mapped to 12p13.31. NANOG is thought to be a key factor in maintaining pluripotency. Moreover, NANOG is also thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. The NANOG protein has been found to be a transcriptional activator for the Rex1 promoter, playing a key role in sustaining Rex1 expression. Knockdown of NANOG in embryonic stem cells results in a reduction of Rex1 expression, while forced expression of NANOG stimulates Rex1 expression.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Nanog Antibody Picoband® catalog # A00153-4. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone m
Host	Rabbit
Immunogen Region	E.coli-derived mouse Nanog recombinant protein (Position: M1-A191).
Isotype	Rabbit IgG
Molecular Weight	37 kDa
Reactivity	Mouse, Rat
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	Homeobox protein NANOG;Homeobox transcription factor Nanog;hNanog;NANOG;
Tissue Specificity	Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. .
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Uniprot	Q80Z64
Gene Id	71950
Gene Name	Nanog
Subcellular Location	Nucleus .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	WB, ICC, IF, Flow Cytometry, ELISA
Description	Weste blot, 0.25-0.5 ug/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 ug/ml, Rat Flow Cytometry (Fixed), 1-3 ug/1x10⁶ cells, Mouse ELISA, 0.1-0.5 ug/ml, -

Reference	1. Chambers, I., Colby, D., Robertson, M., Nichols, J., Lee, S., Tweedie, S., Smith, A. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113: 643-655, 2003. 2. Clark, A. T., Rodriguez, R. T., Bodnar, M. S., Abeyta, M. J., Cedars, M. I., Turek, P. J., Firpo, M. T., Pera, R. A. R. Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent cells and map to chromosome 12p13, a hotspot for teratocarcinoma. Stem Cells 22: 169-179, 2004. 3. Hart, A. H., Hartley, L., Ibrahim, M., Robb, L. Identification, cloning and expression analysis of the pluripotency promotingNanog genes in mouse and human. Dev. Dyn. 230: 187-198, 2004.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003805-1	A00153-4	-	100 ug	$370		Add to Cart
BHA21003805-2	A00153-4-10ug	Sample	10 ug	$99		Add to Cart
BHA21003805-3	A00153-4-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003805-4	A00153-4-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003805-5	A00153-4-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003805-6	A00153-4-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003805-7	A00153-4-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003805-8	A00153-4-FITC	FITC	100 ug	$570		Add to Cart
BHA21003805-9	A00153-4-HRP	HRP	100 ug	$570		Add to Cart
BHA21003805-10	A00153-4-APC	APC	100 ug	$670		Add to Cart
BHA21003805-11	A00153-4-PE	PE	100 ug	$670		Add to Cart
BHA21003805-12	A00153-4-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003805-13	A00153-4-carrier-free	carrier-free	100 ug	$370		Add to Cart

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