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Home / Primary Antibodies / Polyclonal Antibodies

Anti-METTL14 Antibody Picoband®

SKU : BHA21003071

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Mfr.No.	A07389-2, A07389-2-10ug, A07389-2-Biotin, A07389-2-Cy3, A07389-2-Dylight488, A07389-2-Dylight550, A07389-2-Dylight594, A07389-2-FITC, A07389-2-HRP, A07389-2-APC, A07389-2-PE, A07389-2-iFluor647, A07389-2-carrier-free
Alternative Names	mucin 20, cell surface associated
Background	Methyltransferase like 14 is a protein that in humans is encoded by the METTL14 gene. METTL14 is part of a methyltransferase complex required for the common N6-methyladenosine (m6A) base modification that is enriched near stop codons and in long exons of mRNAs.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-METTL14 Antibody Picoband® catalog # A07389-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression.
Host	Rabbit
Immunogen Region	E.coli-derived human METTL14 recombinant protein (Position: Q12-D350).
Isotype	Rabbit IgG
Molecular Weight	65 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Mucin-20; MUC-20; MUC20; KIAA1359; UNQ2782/PRO7170
Tissue Specificity	Highly expressed in kidney, moderately in placenta, lung, prostate, liver, and digestive system. In the kidney, localized in the proximal tubules but not in the glomerulus or distal tubules. Detected in most of the male urogenital tract epithelia, with the exception of epididymis.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

Uniprot	Q9HCE5
Gene Id	57721
Gene Name	METTL14
Subcellular Location	Apical cell membrane. Basolateral cell membrane. Microvillus membrane. Secreted.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	ELISA, Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.25ug/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Hartz, P. A. Personal Communication. Baltimore, Md. 8/4/2015. <br /><br />2. Liu, J., Yue, Y., Han, D., Wang, X., Fu, Y., Zhang, L., Jia, G., Yu, M., Lu, Z., Deng, X., Dai, Q., Chen, W., He, C. A METTL3-METTL14 complex mediates mammalian nuclear RNA N(6)-adenosine methylation. Nature Chem. Biol. 10: 93-95, 2014. <br /><br />3. Nagase, T., Kikuno, R., Nakayama, M., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 273-281, 2000.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003071-1	A07389-2	-	100 ug	$370		Add to Cart
BHA21003071-2	A07389-2-10ug	Sample	10 ug	$99		Add to Cart
BHA21003071-3	A07389-2-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003071-4	A07389-2-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003071-5	A07389-2-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003071-6	A07389-2-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003071-7	A07389-2-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003071-8	A07389-2-FITC	FITC	100 ug	$570		Add to Cart
BHA21003071-9	A07389-2-HRP	HRP	100 ug	$570		Add to Cart
BHA21003071-10	A07389-2-APC	APC	100 ug	$670		Add to Cart
BHA21003071-11	A07389-2-PE	PE	100 ug	$670		Add to Cart
BHA21003071-12	A07389-2-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003071-13	A07389-2-carrier-free	carrier-free	100 ug	$370		Add to Cart

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