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Home / PCR, qPCR & RT-PCR / PCR

Safe-Green™ 1kb Opti-DNA Marker

SKU : BHR10900160

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Applied Biological Materials (abm) Inc.

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Mfr.No.	G474
Description	Safe-Green™ Opti-DNA Markers contain Safe-Green™ DNA stain which is a non-toxic and efficient alternative DNA visualizing agent to the carcinogenic ethidium bromide (EB). The brightness of Safe-Green™ Opti-DNA Markers is optimized for compatible and proper camera exposure in applications along with PCR products generated from abm’s Safe-Green™ 2X PCR MasterMixes as well as from any other SYBR Green-alike-based PCR amplification. To use Safe-Green™ Opti-DNA Markers, simply load 5 ul of the marker onto a plain gel and perform electrophoresis along with samples; Visualize the gel using an UV light source. Safe-Green™ has an excitation maximum at 495 nm and an emission maximum at 520 nm. PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 19 bands, ranging from 100 pb – 10 kb, for molecular weight standards in agarose gel electrophoresis. The 500, 1,500, and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
Suggested-load	Resolves into 19 sharp, tight bands.Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Bands	19 bands
Quantity	500 µl/100 loads
Range	100bp-10kb
Reaction	Marker

Reference	Wang, HY et al. "DNA barcoding of fisheries catch to reveal composition and distribution of cutlassfishes along the Taiwan coast." Fish. Res. :105 (2017). DOI: 10.1016/j.fishres.2016.11.015. Application: DNA Markers.

Storage Condition	-20C
Shipping Condition	Ice Packs

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SKU	Supplier No.	Size	Your price	Quantity
BHR10900160	G474	500 ?l/100 loads	$70		Add to Cart

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