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Home / PCR, qPCR & RT-PCR / PCR Enzymes & Reagents

Plant Tissue Direct PCR Kit (With Dye)

BHR20800043

Plant Tissue Direct PCR Kit (With Dye) can directly amplify different types of plant leaves by PCR, with wide adaptability and strong stability. This direct PCR kit consists of three components, buffer P1, buffer P2 and 2x plant master mix. Among of them, 2x plant master mix is a premix solution containing hot-start Taq DNA polymerase, dNTP mix, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc. Moreover, this plant master mix also includes electrophoresis loading buffer, which can be directly used in electrophoresis after PCR.

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+1 866.986.9598
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[email protected]
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Shipping The products are shipped with ice packs.
Note 1. When doing leaf experiments, it is recommended to use freshly collected leaf tissue. If it is a long-term frozen tissue, it needs to be stored at -80°C. Repeated freezing and thawing should be avoided as much as possible to avoid template degradation and affect PCR efficiency. The leaf tissue is suitable for young leaves. If it is a mature leaf, avoid using the tissue of the main vein of the leaf.

2. It is recommended to amplify the fragment within 1 kb in length for the best amplification efficiency.

3. When sampling, use a hole punch or knife to take a sample of suitable size. When the samples are different, the hole punch or knife needs to be cleaned every time before processing the sample.

4. For leaf tissue, it is recommended to take 1-10 mm leaves, too small leaf length will lead to low PCR amplification yield, too much will inhibit the PCR reaction, use the method of thermal cracking, mashing with a pipette tip, and crushing with a grinder to treat plant leaves. After treatment, it needs to be shaken and centrifuged. Be sure to take the supeatant for testing. Precipitation will seriously inhibit the PCR reaction.

5. For your safety and health, please wear lab coats and disposable gloves for operation.

6. This product is for research use ONLY！
Storage 1. Reagent 10187-A [Buffer P1] can be stored at 4℃ for 1 year.
2. Reagent 10187-B [Buffer P2], used for neutralizing lysate, which is beneficial to store the sample for a longer time, and can be stored at 4℃ for 1 year.
3. Reagent 10187-C [2× Plant Master Mix] can be stored at -20℃ for 1 year. Avoid repeated freezing and thawing.
Manual Safety Data Sheet
​Manual
Combination SKU Supplier No. Size Your price Quantity
BHR20800043-1 10187ES50 50T $85
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