2× Hieff™ PCR Master Mix (No Dye)

Mfr.No.

10103ES03, 10103ES08

Description

2x Hieff^TM PCR Master Mix (No Dye) is a kind of conventional PCR premixed solution which is ready to use. This 2x PCR master mix from Yeasen is composed of HieffTM Taq DNA Polymerase (Cat#10101), dNTP mix, MgCl2 and optimized buffer. Note that, this taq polymerase master mix doesn't contain bromophenol blue dye. Compared to 2x PCR master mix (With Dye) (Cat#10102), its PCR products isn't used to electrophoresis directly. But, during the reaction with this PCR master mix (no dey), only the primer and template can be added for amplification, which greatly simplifies the operation steps of experiment.

Features

Convenient, ready-to-use mix
Thermostable—half life is more than 40 min at 95°C
Generates PCR products with 3'-dA overhangs

Product Components

Components No.	Name	10103ES03	10103ES08	10103ES60
10103	2×Hieff™ PCR Master Mix (With Dye)	1 mL	5×1 mL	100×1 mL

Specifications

Fidelity (vs. Taq)	1 ×
Hot Start	No
Overhang	3'-A
Polymerase	Taq DNA Polymerase
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Product Type	PCR Master Mix (2×)

Reference	[1] Liu Y, Lai M, Li S, et al. LS-106, a novel EGFR inhibitor targeting C797S, exhibits antitumor activities both in vitro and in vivo. Cancer Sci. 2022;113(2):709-720. doi:10.1111/cas.15229(IF:6.716)

Storage	The 2×Hieff™ PCR Master Mix products should be stored at -25°C~-15℃ for 2 years.

Manual	Manual Safety Data Sheett

No Description Available.

The expected 1.2 kb PCR products can be amplified with 2×Hieff™ PCR Master Mix. The Master Mix was stored at-20°C for 1 year following another 3 months at 4°C and 1 month at 25°C. Template: Arabidopsis genome. Annealing temperature: 60°C. Extension time: 40 sec.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHR20800003-1	10103ES03	1mL	$15		Add to Cart
BHR20800003-2	10103ES08	5x1mL	$65		Add to Cart

2× Hieff™ PCR Master Mix (No Dye)

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