2× Hieff™ HotStart PCR Genotyping Master Mix (With Dye)

Mfr.No.

10108ES03, 10108ES50, 10108ES08

Description

2xHieff^TM HotStart PCR Genotyping Master Mix (With Dye) is a ready-to-use PCR pre-mixed solution containing Hieff^TM HotStart Taq DNA Polymerase, dNTPs, and an optimized buffer system. It simplifies experimental procedures significantly by requiring only the addition of primers and template for amplification, enabling high-throughput operations and improving reproducibility of experimental results. Hieff^TM HotStart Taq DNA Polymerase is a ligand-modified thermostable Taq DNA Polymerase whose activity is modulated with temperature changes. Its enzyme activity is completely blocked at room temperature and released upon heating at 95°C. Activation of Hieff^TM HotStart DNA Polymerase requires only 2-3 minutes and is compatible with existing PCR programs. This product prevents non-specific amplification during sample preparation and temperature cycling stages, making it effective for genotyping experiments.

Features

Convenient, ready-to-use mix
Thermostable—half-life is more than 40 min at 95°C
Generates PCR products with 3'-dA overhangs

Product Components

Name	10108ES03	10108ES08	10108ES50	10108ES60
2× Hieff™ HotStart PCR Genotyping Master Mix(With Dye)	1 mL	5×1 mL	50×1 mL	100×1 mL

PCR Reaction（50 μL）

Components	Vol.	Conc.
ddH2O	to 50 μL	-
DNA	/	-
Primer F (10 μM)	2 μL	0.4 μM
Primer R (10 μM)	2 μL	0.4 μM
2×Hieff™ HotStart PCR Genotyping Master Mix (With Dye)	25 μL	1×

DNA Type	Input
Genomic DNA	50 ng-100 ng
Plasmids	100 pg-20 ng
cDNA	1-5 μL

PCR Cycling

Temp.	Time	Cycles
95°C	5 min	1
95°C	30 sec	35
50-60°C	30 sec
72°C	30 sec/kb
72°C	10 min	1

Specifications

Fidelity (vs. Taq)	1 ×
Hot Start	No
Overhang	3'-A
Polymerase	Taq DNA Polymerase
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Product Type	PCR Master Mix (2×)

Applications

Genotyping PCR, HotStart PCR

Reference	[1] Han P, Cao P, Yue J, et al. Knockdown of hnRNPA1 Promotes NSCLC Metastasis and EMT by Regulating Alteative Splicing of LAS1L exon 9. Front Oncol. 2022;12:837248. Published 2022 Jun 23. doi:10.3389/fonc.2022.837248(IF:5.738)

Shipping	Shipped on ice packs.
Storage	Store at -20°C with a shelf life of 2 years.

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Combination SKU	Supplier No.	Size	Your price
BHR20800002-1	10108ES03	1mL 100T	$18	Add to Cart
BHR20800002-2	10108ES50	50x1mL 5000T	$590	Add to Cart
BHR20800002-3	10108ES08	5x 1mL 500T	$80	Add to Cart

2× Hieff™ HotStart PCR Genotyping Master Mix (With Dye)

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