SafeView Plus™ represents the newest generation of nucleic acid stains. It can be safely and widely used for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose and polyacrylamide gels. SafeView Plus™ was developed to replace toxic ethidium bromide (EB, a potent mutagen) and has higher sensitivity and enhanced performance than SafeView™.
SafeView Plus™ is used as a substitute for ethidium bromide for post
electrophoresis DNA staining. There is no need to add it to the
electrophoresis buffer or casting gel. The fluorescence has an
excitation maxima at approximately 490nm.
1. Prepare staining solution by diluting SafeView Plus™ 1:5,000 -
1:10,000 in TE, TAE or TBE buffer.
2. After performing electrophoresis, place the dye-free gel in a plastic
container and
cover with the staining solution. Ensure the box is covered to protect
the contents
from light. Agitate gently and incubate at room temperature for 10-40
minutes.
3. No destaining is required - visualize results directly under UV light.