OneScript Plus Reverse Transcriptase (PCR)

Mfr.No.	G237
Description	Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with abm’s EasyScript™ Plus Reverse Transcriptase. Engineered to perform under high temperatures (45°C - 55°C), abm’s EasyScript™ Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, EasyScript Plus™ Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.
Template	RNA
Trade Marker	OneScript®
Buffer	Enzyme supplied with 5X RT buffer
Concentration	200 U/ul
Quantity	25 x 20 µl reactions

Reference	Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR. Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modelling." J Physiol 593(2):365-383 (2015). DOI: 10.1113/jphysiol.2014.284109. PubMed: 25630259. Application: Reverse Transcription. Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular networkmodeling." J Physiol. : (2014). PubMed: 25384784. Application: Reverse Transcription. Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR. Kim, SJ et al. "Selective expression of transgene using hypoxia-inducible trans-splicing group I intron ribozyme." J Biotechnol. Pt A:22-7 (2014). DOI: 10.1016/j.jbiotec.2014.10.001. PubMed: 25312327. Application: Reverse Transcription. Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription. Meng, X et al. "Rapid and efficient reprogramming of human fetal and adult blood CD34+ cells into mesenchymal stem cells with a single factor." Cell Res. 23:658-672 (2013). DOI: 10.1038/cr.2013.40. Application: Reverse Transcription. Menon, V et al. "The contribution of visceral fat to improved insulin signaling in Ames dwarf mice." Aging Cell 13 (3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription. Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.