5X All-In-One RT MasterMix

Mfr.No.	G592
Description	Convenient and time-saving reaction set-up with abm’s cDNA Synthesis SuperMix. abm’s cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, abm’s cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. Key Features • Streamlined protocol suitable for high-throughput applications • Ease of use with a simple set-up • Excellent cDNA yield This SuperMix contains abm's EasyScript™ Reverse Transcriptase .
Template	RNA
Trade Marker	OneScript®
Buffer	RTase is in storage buffer containing 50 % glycerol.
Format	SuperMix
Quantity	25 x 20 µl reactions
Reaction	RT-PCR

Reference	Amodio, et al. "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach." Cell Biology and Toxicology 32:285 (2016). DOI: 10.1007/s10565-016-9335-z. Application: Luciferase Assay. Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR. Dahiya , S et al. "Sequence analysis of the Toll-like receptor 2 gene of old world camels ." J. Adv. Res. : (2013). DOI: 10.1016/j.jare.2013.09.001. Application: Reverse Transcription. Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR. Kim, B.H et al. "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1-and Th17-cell-mediated immune responses." Biochem. Pharmacol. :45-57 (2016). DOI: http://dx.doi.org/10.1016/j.bcp.2016.03.018. Kim, BH et al. "Sophoraflavanone G induces apoptosis of human cancer cells by targeting upstream signals of STATs." Biochem. Pharmacol. 86(7):950-959 (2013). DOI: 10.1016/j.bcp.2013.08.009. Application: Reverse Transcription. Lee, G et al. "Cancer cell expressions of immunoglobulin heavy chains with unique carbohydrate-associated biomarker." Cancer Biomark. 5(4):177-188 (2009). DOI: 10.3233/CBM-2009-0102. Application: Reverse Transcription. Lee, G et al. "Carbohydrate-associated Immunodominant Epitope(s) of CA215." Immunol. Invest. 41(3):317-336 (2012). DOI: 10.3109/08820139.2011.633141. Application: Reverse Transcription. Lee, G et al. "Growth inhibition of tumor cells in vitro by using monoclonal antibodies against gonadotropin-releasing hormone receptor." Cancer Immunol. Immunother. 59(7):1011-1019 (2010). DOI: 10.1007/s00262-010-0823-3. Application: Reverse Transcription. Lee, G et al. "Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies." Cancer Immunol. Immunother. 59(9):1347-1356 (2010). DOI: 10.1007/s00262-010-0864-7. Application: Reverse Transcription. Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription. Lee, G et al. "Widespread expressions of immunoglobulin superfamily proteins in cancer cells." Cancer Immunol. Immunother. 61(1):89-99 (2012). DOI: 10.1007/s00262-011-1088-1. Application: Reverse Transcription. Nagarajan, G et al. "Cloning and phylogenetic analysis of Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) from Indian dromedaries (Camelus dromedarius)." Comp. Immunol. Microbiol. Infect. Dis. 34(3):291-298 (2011). DOI: 10.1016/j.cimid.2011.01.005. Application: Reverse Transcription. Nagarajan, G et al. "Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius)." Res. Vet. Sci. 92(3):420-426 (2012). DOI: 10.1016/j.rvsc.2011.03.028. Application: Reverse Transcription. Tang, Y et al. "Similar Gene Regulation Patterns for Growth Inhibition of Cancer Cells by RP215 or Anti-Antigen Receptors." J. Cancer Sci. Ther. 5:200-208 (2013). DOI: 10.4172/1948-5956.1000207 . Application: Reverse Transcription. Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.