Choose your priming methods with abm’s cDNA Synthesis Kit. abm’s cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.
Key Features
• Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
• Robust cDNA synthesis from any RNA template
• High reproducibility and excellent yield
This kit employs abm's EasyScript™ Reverse Transcriptase .
Template
RNA
Trade Marker
OneScript®
Buffer
In storage buffer containing 50 % glycerol.
Concentration
200 U/ul
Format
Kit
Quantity
100 x 20 µl reactions
Reaction
RT-PCR
Reference
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Monaco, A et al. "Neuroglial alterations in the zebrafish brain exposed to cadmium chloride." J. Appl. Toxicol. 12:1629-1638 (0). DOI: 10.1002/jat.3328.
Nagarajan, G et al. "Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius)." Res. Vet. Sci. 92 (3):420-426 (2012). DOI: 10.1016/j.rvsc.2011.03.028. PubMed: 21529863. Application: Reverse Transcription.
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Prakhar, P et al. "Ac2PIM-responsive miR-150 and miR-143 Target Receptor-interacting Protein Kinase 2 and Transforming Growth Factor Beta-activated Kinase 1 to Suppress NOD2-induced Immunomodulators." J Biol Chem 44:26576-26586 (2015). DOI: 10.1074/jbc.M115.662817. PubMed: 26391398.
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Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.
Wood, L et al. "Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome." Sci. Rep. : (0). DOI: 10.1038/srep19857.
Zhang, H et al. "IKKϵ negatively regulates RIG-I via direct phosphorylation." J Med Virol : (2015). DOI: 10.1002/jmv.24376. PubMed: 26354181. Application: Reverse Transcription.