Choose your priming methods with abm’s cDNA Synthesis Kit. abm’s cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alteatives to gene-specific primers.
Key Features
• Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
• Robust cDNA synthesis from any RNA template
• High reproducibility and excellent yield
This kit employs abm's EasyScript™ Reverse Transcriptase .
Template
RNA
Trade Marker
OneScript®
Format
Kit
Quantity
25 x 20 µl reactions
Reaction
RT-PCR
Reference
Afroz, S et al. ""Mesoporous ZnO nanocapsules for the induction of enhanced antigen-specific immunological responses"." Annu. Rep. R. Soc. Chem. :14641-14653 (2017). DOI: 10.1039/c7nr03697c. Application: RT-PCR.
Akcilar, R et al. "The effect of adropin on lipid and glucose metabolism in rats with hyperlipidemia." Iran. J. Basic. Med. Sci. 3:245-251 (0). PubMed: PMC4834113.
Baylink, D et al. "Systemic and local ex vivo gene therapy of the skeleton ." U.S. Patent No. 20, 160, 120, 944 : (0).
Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
Correnti, JM et al. "Pharmacological ceramide reduction alleviates alcohol-induced steatosis and hepatomegaly in adiponectin knockout mice." Am J Physiol Gastrointest Liver Physiol 306(11):G959-73 (2014). DOI: 10.1152/ajpgi.00395.2013. PubMed: 24742988. Application: Reverse Transcription.
Dahiya, SS et al. "Sequence analysis of the Toll-like receptor 2 gene of old world camels." Joual of Advanced Research : (2013). DOI: 10.1016/j.jare.2013.09.001. Application: Reverse Transcription.
Dia, G et al. "MicroRNA-146a knockdown suppresses the progression of ankylosing spondylitis by targeting dickkopf 1." Biomed. Pharmacother. :1243-1249 (2017). DOI: 10.1016/j.biopha.2017.11.067. Application: Reverse transcription.
Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
Kaam, A et al. "Sonic hedgehog-responsive lipoxygenases and cyclooxygenase-2 modulate Dectin-1-induced inflammatory cytokines." Mol Immunol 68(2 Pt A):280-9 (2015). DOI: 10.1016/j.molimm.2015.09.013. PubMed: 26432261. Application: Reverse Transcription.
Kim, SJ et al. "Targeting the MicroRNA Passenger Strand for Regulating Therapeutic Transgenes." Nucleic Acid Ther 25(4):209-18 (2015). DOI: 10.1089/nat.2015.0543. PubMed: 26076094. Application: Reverse Transcription.
Lee, G et al. "Cancer cell expressions of immunoglobulin heavy chains with unique carbohydrate-associated biomarker." Cancer Biomark 5(4):177-188 (2009). DOI: 10.3233/CBM-2009-0102.
Lee, G et al. "Carbohydrate-associated Immunodominaant Epitope(s) of CA215." Immunol. Invest. 41(3):317-336 (2012). DOI: 10.3109/08820139.2011.633141.
Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268. Application: Reverse Transcription.
Lee, G et al. "Widespread expressions of immunoglobulin superfamily proteins in cancer cells." Cancer Immunol Immunother 61(1):89-99 (2012). DOI: 10.1007/s00262-011-1088-1. PubMed: 21850469.
Li, R et al. "Ultrafine particles from diesel vehicle emissions at different driving cycles induce differential vascular pro-inflammatory responses: implication of chemical components and NF-kappaB signaling." Part Fibre Toxicol 7:6 (2010). DOI: 10.1186/1743-8977-7-6. PubMed: 20307321. Application: Reverse Transcription.
Monaco, A et al. "Neuroglial alterations in the zebrafish brain exposed to cadmium chloride." J. Appl. Toxicol. 12:1629-1638 (0). DOI: 10.1002/jat.3328.
Nagarajan, G et al. "Cloning and phylogenetic analysis of Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) from Indian dromedaries (Camelus dromedarius)." Comp. Immunol. Microbiol. Infect. Dis. 34(3):291-298 (2011). DOI: 10.1016/j.cimid.2011.01.005.
Nagarajan, G et al. "Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius)." Res. Vet. Sci. 92 (3):420-426 (2012). DOI: 10.1016/j.rvsc.2011.03.028. PubMed: 21529863. Application: Reverse Transcription.
Park, H-S et al. "PPARγ neddylation essential for adipogenesis is a potential target for treating obesity." Cell Death Diff. : (0). DOI: 10.1038/cdd.2016.6. Application: cDNA Synthesis .
Prakhar, P et al. "Ac2PIM-responsive miR-150 and miR-143 Target Receptor-interacting Protein Kinase 2 and Transforming Growth Factor Beta-activated Kinase 1 to Suppress NOD2-induced Immunomodulators." J Biol Chem 44:26576-26586 (2015). DOI: 10.1074/jbc.M115.662817 . PubMed: 26391398.
Ramraj, SK et al. "Serum-circulating miRNAs predict neuroblastoma progression in mouse model of high-risk metastatic disease." Oncotarget 14:18605-18619 (0).
Rosarita , T et al. "Simvastatin inhibits the expression of stemness‑related genes and the metastatic invasion of human cancer cells via destruction of the cytoskeleton." Int. J. Oncol. :1851-1859 (2017). DOI: 10.3892/ijo.2017.4158. Application: RT-PCR.
Tang, Y et al. "Similar gene regulation pattes for growth inhibition of cancer cells by RP215 or anti-antigen receptors." Joual of Cancer Science &Therapy 5:200-208 (2013). DOI: 10.4172/1948-5956.1000207. Application: Reverse Transcription.
Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.
Wood, L et al. "Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome." Sci. Rep. : (0). DOI: 10.1038/srep19857. Application: cDNA Synthesis .
Zhang, X et al. "IKKϵ negatively regulates RIG-I via direct phosphorylation." J Med Virol : (2015). DOI: 10.1002/jmv.24376. PubMed: 26354181. Application: Reverse Transcription.