Hifair™ V Reverse Transcriptase

Mfr.No.	11300ES93, 11300ES92
Description	Hifair^TM Advanced One Step RT-qPCR SYBR Green Kit is a kit for fluorescence quantification based on SYBR Green I dye. Using gene-specific primers, reverse transcription and qPCR reactions are completed in one tube, eliminating the need for repeated cap-opening and pipetting operations, greatly improving assay efficiency and reducing the risk of contamination. For RNA samples, the kit employs heat-resistant Hifair^TM V Reverse Transcriptase for efficient cDNA synthesis and UNICON^TM HotStart Taq DNA Polymerase for quantitative amplification. Under the optimized buffer system, the sensitivity of the kit can be as high as 0.1 pg for highly expressed targets and as high as 1 pg for moderately expressed targets, and the kit is also suitable for amplification and quantification of DNA samples. The kit is suitable for the amplification and quantification of DNA samples. It enables the sensitive detection and quantification of nucleic acids from different plant and animal samples, cells and microorganisms.
Features	Outstanding thermal resistance. Suitable for RT-PCR/RT-qPCR applications. Available in various forms including high and low concentrations, and glycerol-free, to meet diverse requirements.

Manual	11300-A-MSDS-HB220419.pdf 11300-B-MSDS-HB220419.pdf Manual

Capable of withstanding a reaction temperature of 58°C, it is suitable for the reverse transcription of complex RNA templates. The Reverse Transcriptase of Hifair™ V Reverse Transcriptase and the brand T* were incubated at 55℃ and 58℃ respectively for 30min to detect the residual enzyme activity. The results showed that Hifair™ V Reverse Transcriptase could still maintain 70% activity after incubation at 58℃ for 30min, which was much higher than that of the brand T*.

The detection rate of RT-qPCR was better than that of other brands, and the detection range was 1 pg-1 μg. Using 1 pg-1 μg (7 gradients) total RNA of 293T cells as template, reverse transcription was performed using Hifair™ V one-step RT-gDNA digestion SuperMix for qPCR(Cat#11142), V*, T*. The obtained cDNA was quantitatively analyzed by qPCR. The results showed that the sensitivity of 11142ES could reach 1 pg, which was better than that of other brands.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHR20800038-1	11300ES93	5x10000U	$1530		Add to Cart
BHR20800038-2	11300ES92	10000U	$380		Add to Cart

Hifair™ V Reverse Transcriptase

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