Hieff UNICON™ Hotstart E-Taq DNA Polymerase, 5 U/μL

Mfr.No.	10726ES92, 10726ES72, 10726ES80
Description	Hieff UNICON^TM Hotstart E-Taq DNA Polymerase is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'?3' polymerase activity of Taq DNA polymerase, but also blocks the 5'?3' exonuclease activity. Heating for 32 seconds at the pre-denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature.
Features	High Specificity: Capable of dual encapsulation, it simultaneously inhibits the 5'-3' Taq DNA polymerase activity and the 5'-3' exonuclease activity at room temperature, enhancing the specificity of the reaction. No Nuclease Contamination: Free from contamination of exonucleases, endonucleases, and RNases. Suitable for High-Sensitivity PCR Experiments: Exhibits a good linear relationship when the template concentration ranges from 5×10^6 copies/T to 5×10^1 copies/T, with the lowest detection limit reaching 5 copies/T. Superior Stability: When formulated into a complete premix containing primers and probes, it remains stable after being left at 37°C for 14 days and after 50 freeze-thaw cycles. Compatible with Rapid Amplification Protocols: 45 cycles, results within 30 minutes.
Applications	HotStart PCR

Note	Yeasen Biotech, with years of experience in the field of enzyme raw materials, has been providing high-quality and cost-effective molecular diagnostic PCR raw materials to IVD companies, third-party testing institutions, and research organizations. These include single components such as reverse transcriptase, RNase inhibitor, Taq DNA polymerase, uracil DNA glycosylase (UDG/UNG enzyme), Taq enzyme antibody, dNTPs, as well as system-optimized qPCR/RT-qPCR premix solutions, offering superior performance and stable enzyme raw materials for the development of clinical/non-clinical diagnostic kits.

Manual	Safety Data Sheet Manual

Figure shows that the qPCR master mix prepared with 10726 exhibits good linearity when the template concentration ranges from 5×10^6 copies/T to 5×10^1 copies/T，R2=0.9997.

Demonstrates the stability performance of qPCR master mix prepared with 10726ES, using ASFV plasmid DNA as a template under various temperature and time conditions. The results indicate that the qPCR master mix formulated with 10726ES maintains stable performance.

Figure shows that the qPCR master mix prepared with 10726ES, using ASFV plasmid DNA as a template, can stably amplify and detect the limit concentration of template DNA（5 copies/T） under both standard（80min） and fast（30min） amplification programs.

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Combination SKU	Supplier No.	Size	Your price
BHR20800013-1	10726ES92	10000 U	$1415	Add to Cart
BHR20800013-2	10726ES72	250 U	$55	Add to Cart
BHR20800013-3	10726ES80	1000 U	$185	Add to Cart

Hieff UNICON™ Hotstart E-Taq DNA Polymerase, 5 U/μL

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