Hieff UNICON™ Hotstart Direct DNA Polymerase, 50 U/μL

Mfr.No.

10718ES01, 10718ES00

Description

Hieff UNICON^TM Hotstart E-Taq DNA Polymerase is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'?3' polymerase activity of Taq DNA polymerase, but also blocks the 5'?3' exonuclease activity. Heating for 31 seconds at the pre-denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature.

Product Components

Components No.	Name	10718ES00 (500 U)	10718ES01 (5 KU)	10718ES94 (50 KU)	10718ES95 (1250 KU)
10718	Hotstart D-Taq (50 U/μL)	10 μL	100 μL	1 mL	25 mL

Product Details

Reaction Composition

Components	Volume (μL)	Final Concentration
2 × Buffera	25	1 ×
Primer/Probe Mixb	X	0.1 -0.5 μM
Hotstart D-Taq (50 U/μL)c	0.12	0.12 U/μL
Template DNAb	X	0.1-100 ng
ddH2O	to 50	-

Note：Be sure to mix well before use, avoid excessive bubbles caused by violent vibration.
a) According to the specific experimental application, it is needed to prepare the corresponding reaction buffer. If a basic buffer (10×) is required, Cat#11374 is recommended.
b) The amount of DNA and the concentration of probes or primers are recommended concentrations. The optimal concentration can be adjusted according to the specific experimental conditions.
c) The amount of enzyme can be adjusted according to the experimental application.

Optimized Cycling Protocol

	Reaction stage	Temperature	Time	Cycle
1	Initial denaturation	95°C	5 min	1
2	Amplification reaction	95°C	15 sec	45 cycles
2	Amplification reaction	60°Ca	30 secb	45 cycles

Note：
a) Amplification reaction: The temperature is adjusted according to the Tm value of the designed primers.
b) Fluorescence signal acquisition: Please set the experimental procedure according to the requirements of the instrument manual.

Applications

HotStart PCR, Direct PCR

Storage	The product should be stored at -20°C for 2 years.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHR20800011-1	10718ES01	5000 U	$1200		Add to Cart
BHR20800011-2	10718ES00	500 U	$150		Add to Cart

Hieff UNICON™ Hotstart Direct DNA Polymerase, 50 U/μL

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