ExCellenCT One-Step TaqProbe qRT-PCR-iCycler

Mfr.No.	G918-PC
Description	Extracting and preparing RNA templates directly from cultured cells to be applied as template for One-Step qRT-PCR, with G493 One-Step TaqProbe qRT-PCR MasterMix. abm’s ExCellenCT Lysis Kit is an unique and effective alternative method to provide RNA templates that are extracted and processed directly from cultured cells in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template to be applied in One-Step qRT-PCR. The One-Step TaqProbe qRT-PCR system uses a combination of high-quality enzymes in a proprietary buffer system to deliver precise and accurate sample analysis with high sensitivity and superb signal-to-noise ratio. The use of HotStart Taq DNA Polymerase in the enzyme mix significantly reduces non-specific PCR amplification. Coupled together, this complete system provides the ultimate convenience in generating consistent, reproducible, and accurate results from 10-10⁵ cells.
Template	RNA
Tested Machines	BioRad™ iCycler™, BioRad™ iQ™5, BioRad™MyiQ™
Detection Chemistry	TaqProbe
Quantity	25 Preps 100 x 20 µl reactions
Reaction	Onestep qRTPCR

Reference	Chi, M et al. "Reduced polyphenol oxidase gene expression and enzymatic browning in potato (Solanum tuberosum L.) with artificial microRNAs." BMC Plant Biol. 14:62 (2014). DOI: 10.1186/1471-2229-14-62. PubMed: 24618103. Application: qRT-PCR. Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR. Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR. Li, R et al. "Angiopoeitin-2 modulates Survivin expression in OxLDL-induced endothelial cell apoptosis." Biochem. Biophys. Res. Commun. 417 (1):619-622 (2012). DOI: 10.1016/j.bbrc.2011.12.018. PubMed: 22182412. Application: qRT-PCR. Lin, C et al. "Root hair-specific EXPANSIN A7 is required for root hair elongation in Arabidopsis." Mol. Cells 31 (4):393-397 (2011). DOI: 10.1007/s10059-011-0046-2. PubMed: 21359675. Application: qPCR. Moazzen, H et al. "N-Acetylcysteine prevents congenital heart defects induced by pregestational diabetes." Cardiovasc Diabetol 13:46 (2014). DOI: 10.1186/1475-2840-13-46. PubMed: 24533448. Application: qRT-PCR.

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SKU	Supplier No.	Size	Your price	Quantity
BHR10900138	G918-PC	25Preps	$135		Add to Cart