Problems with non-specific amplification and primer-dimer formation? Try abm’s HotStart DNA Polymerase. HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, abm’s HotStart DNA Polymerase offers improved yield of desired PCR products.
With abm’s HotStart DNA Polymerase, you can achieve
• The highest specificity with minimal background
• Superior performance
• Improved yield of desired product
Template
DNA
Buffer
Enzyme supplied with 10X buffer and MgSO4
Concentration
5 U/µl
Format
Polymerase
Hot Start Pcr
HotStart
Quantity
250 U (50 µl)
Reaction
PCR
Reference
Ahn, CH et al. "Bacterial biofilm-community selection during autohydrogenotrophic reduction of nitrate and perchlorate in ion-exchange brine." Appl. Microbiol. Biotechnol. 81 (6):1169-1177 (2009). DOI: 10.1007/s00253-008-1797-3. PubMed: 19066883. Application: PCR.
Bhagwat, B et al. "An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation." J Genet Genomics 40 (5):261-270 (2013). DOI: 10.1016/j.jgg.2013.03.012. PubMed: 23706301. Application: PCR.
Chaiyakul, M et al. "Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus." J. Virol. 84 (21):11440-11447 (2010). DOI: 10.1128/JVI.01030-10. PubMed: 20810737. Application: PCR.
Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174-1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
Lee, G et al. "Cancer cell expressions of immunoglobulin heavy chains with unique carbohydrate-associated biomarker." Cancer Biomark 5 (4):177-188 (2009). DOI: 10.3233/CBM-2009-0102. PubMed: 19729827. Application: PCR.
Lee, G et al. "Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies." Cancer Immunol. Immunother. 59 (9):1347-1356 (2010). DOI: 10.1007/s00262-010-0864-7. PubMed: 20473495. Application: PCR.
Paulson, A.R et al. "Transcriptome analysis provides insight into venom evolution in a seed-parasitic wasp, Megastigmus spermotrophus." Insect Mol. Biol. 5:604-616 (2016). DOI: 10.1111/imb.12247.