5X All-In-One RT MasterMix

Mfr.No.	G592
Description	Removal of contaminating genomic DNA from your samples to reverse transcription of RNA in 30 minutes, with 5X All-In-One RT MasterMix (AccuRT Genomic DNA Removal Kit included). abm’s 5X All-in-One RT MasterMix (with AccuRT Genomic DNA Removal Kit) offers a convenient and complete system to facilitate the complete elimination of contaminating genomic DNA in RNA preparations for rapid synthesis of high-quality cDNA for downstream applications. Key Features • Efficient ds and ssDNA digestion in under 10 minutes • Maximal RNA protection – No heating or organic extraction steps required • Fewer handling errors, with only 1 liquid transfer step for reverse transcription • Streamlined protocol suitable for high-throughput applications • Simple set-up for any RNA template • High reproducibility and excellent cDNA yield Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
Template	RNA
Format	MasterMix
Quantity	100 x 20 µl reactions
Reaction	RT-PCR

Reference	Bradley, R et al. "Glucagon-Like Peptide-2 Requires a Full Complement of Bmi-1 for Its Proliferative Effects in the Murine Small Intestine - See more at: http://press.endocrine.org.proxy.lib.sfu.ca/doi/10.1210/en.2016-1127#sthash.UyebzBXc.dpuf." Endocrinology 7: (2016). DOI: http://dx.doi.org.proxy.lib.sfu.ca/10.1210/en.2016-1127#sthash.UyebzBXc.dpuf. Application: Reverse Transcription. Bradley, R et al. "Glucagon-like peptide-2 requires a full-complement of Bmi-1 for its proliferative effects in the murine small intestine." Endocrinology 7: (2016). DOI: http://dx.doi.org/10.1210/en.2016-1127 . Application: Reverse Transcription. Chen, J et al. "The combination of Tet1 with Oct4 generates high-quality mouse-induced pluripotent stem cells." Stem Cells 33(3):686-98 (2015). DOI: 10.1002/stem.1879. PubMed: 25331067. Application: Reverse Transcription. Chen, L et al. "Snail Driving Alternative Splicing of CD44 by ESRP1 Enhances Invasion and Migration in Epithelial Ovarian Cancer." Cellular Physiology and Biochemistry :2489-2504 (2017). DOI: 10.1159/000484458. Application: RT-PCR. Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR. Dai, X et al. "External Ca 2+ regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." Exp. Cell. Res. : (2016). DOI: http://dx.doi.org/10.1016/j.yexcr.2016.11.004. Application: Reverse Transcription. Deng, H et al. "An efficient polyethylene glycol-mediated transformation system of lentiviral vector in Shiraia bambusicola." Process Biochemistry 10:1357-1362 (2016). DOI: http://dx.doi.org/10.1016/j.procbio.2016.07.013. Application: Reverse Transcription. Deng, H et al. "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." Gene 1:67-72 (2016). DOI: http://dx.doi.org/10.1016/j.gene.2016.01.019. Application: Reverse Transcription. Gao, C et al. "Enhanced metabolic process to indole alkaloids in Clematis terniflora DC. after exposure to high level of UV-B irradiation followed by the dark." BMC Plant Biology 1:231 (2016). DOI: 10.1186/s12870-016-0920-3. Application: Reverse Transcription. Gao, R et al. "Purification, characterization and gene analysis of a new α-glucosidase from shiraia sp. SUPER-H168." Annals of Microbiology :1-13 (2016). DOI: 10.1007/s13213-016-1238-y. Application: Reverse Transcription. Han, X et al. "Intracerebroventricular injection of RFRP-3 delays puberty onset and stimulates growth hormone secretion in female rats." Reproductive Biology and Endocrinology 35: (2017). DOI: 10.1186/s12958-017-0254-5. Application: PCR. Hanson, M et al. "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." BioMed Central 1:228 (2016). DOI: 10.1186/s12862-016-0805-y. Application: Reverse Transcription. Hu, et al. "SWATH-based quantitative proteomics reveals the mechanism of enhanced Bombyx mori nucleopolyhedrovirus-resistance in silkworm reared on UV-B treated mulberry leaves." Proteomics 13-14:1-16 (2017). DOI: 10.1002/pmic.201600383. Application: RT-PCR. Inglese, C et al. "The role of vitamin D signalling in the interaction between mesenchymal mammary tumour cells and macrophages." Thesis : (0). DOI: http://hdl.handle.net/1974/14828. Application: Reverse Transcription. Jeon, H.J et al. "Comparison of the functional characterization between alkane monooxygenases for low-molecular-weight polyethylene biodegradation." Int. Biodeterior. Biodegrad. :202-208 (2016). DOI: http://dx.doi.org/10.1016/j.ibiod.2016.06.012. Application: Reverse Transcription. Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR. Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription. Li, C et al. "PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis." J. Plant Growth Regul. 1:489-500 (2017). DOI: 10.1007/s10725-017-0314-z. Application: PCR. Li, L et al. "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." Int. Immunopharmacol. :189-198 (2016). DOI: http://dx.doi.org/10.1016/j.intimp.2016.02.019. Application: Reverse Transcription. Liu, F et al. "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING‐LIKE 1 (OsCOLE1) and OsCOLE1‐INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." New Phytologist 1:96-107 (2016). DOI: 10.1111/nph.14021. Application: Reverse Transcription. Liu, J.C et al. "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." International Journal of Cardiology :388-393 (2016). DOI: http://dx.doi.org/10.1016/j.ijcard.2016.11.202. Application: Reverse Transcription. Song, J et al. "Mangiferin protects mitochondrial function by preserving mitochondrial hexokinase-II in vessel endothelial cells." BBA 7:1829-1839 (2017). DOI: 10.1016/j.bbadis.2017.05.001. Application: PCR. Su, X et al. "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." Int. Immunopharmacol. :16-25 (2016). DOI: http://dx.doi.org/10.1016/j.intimp.2016.05.005. Application: Reverse Transcription. Sun, M et al. "Hypoxia inducible factor‐1α‐induced IL‐33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." Clinical & Experimental Immunology : (2016). DOI: 10.1111/cei.12896. Application: Reverse Transcription. Suzhen, Q et al. "Neonicotinoid insecticides imidacloprid, guadipyr, and cycloxaprid induce acute oxidative stress in Daphnia magna." Ecotoxicology and Environmental Safety :352-358 (2017). DOI: 10.1016/j.ecoenv.2017.10.042. Application: RT . Tuerdi, N et al. "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." Nanomed. Nanotechnol. Biol. Med. 7:1899-1907 (2016). DOI: http://dx.doi.org/10.1016/j.nano.2016.05.002. Application: Reverse Transcription. Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription. Wang, A et al. "Identification of IFITM1 and IFITM3 in Goose: Gene Structure, Expression Patterns, and Immune Reponses against Tembusu Virus Infection." Biomed Res. Int. : (2017). DOI: 10.1155/2017/5149062. Application: PCR. Wang, Q et al. "Genome-wide analysis and functional prediction of long noncoding RNAs in mouse uterus during the implantation window." Oncotarget 48:84360-84372 (2017). DOI: 10.18632/oncotarget.21031. Application: RT-PCR. Wang, Y et al. "CD55 and CD59 expression protects HER2‑overexpressing breast cancer cells from trastuzumab‑induced complement‑dependent cytotoxicity." Oncology Letters 3:2961-2969 (2017). DOI: 10.3892/ol.2017.6555. Application: RT . Wang, Y et al. "Taurine zinc solid dispersions attenuate doxorubicin-induced hepatotoxicity and cardiotoxicity in rats." Toxicol Appl Pharmacol 289(1):1-11 (2015). DOI: 10.1016/j.taap.2015.08.017. PubMed: 26335259. Application: Reverse Transcription. Yan, B et al. "GoTLR7 but not GoTLR21 mediated antiviral immune responses against low pathogenic H9N2 AIV and Newcastle disease virus infection." Immunology Letters :43266 (2017). DOI: 10.1016/j.imlet.2016.11.001. Application: PCR. Yang, S et al. "Natural Infection of Avian Hepatitis E Virus and Marek's Disease Virus in Brown Layer Chickens in China." Avian Diseases 3:698-704 (2016). Application: Reverse Transcription. Ye, W et al. "Identification and characterization of a novel sesquiterpene synthase from Aquilaria sinensis: An important gene for agarwood formation." Int. J. Biol. Macromol. : (2017). DOI: 10.1016/j.ijbiomac.2017.10.183. Application: PCR. Yu, M et al. "Inhibitory effects of doxycycline on the onset and progression of abdominal aortic aneurysm and its related mechanisms." The American Phytopathological Society :101-109 (2017). DOI: 10.1016/j.ejphar.2017.05.041. Application: PCR. Zhao, G et al. "Characterization of the CDK5 gene in Apis cerana cerana (AccCDK5) and a preliminary identification of its activator gene, AccCDK5r1." Cell Stress and Chaperones :1-16 (2017). DOI: 10.1007/s12192-017-0820-y. Application: RT-PCR. Zhou, H et al. "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." Scientific reports :25545 (2016). DOI: 10.1038/srep25545. Application: Reverse Transcription. Zhou, H et al. "LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies." Frontiers in microbiology :166 (2016). DOI: 10.3389/fmicb.2016.00166. Application: Reverse Transcription. Zhu, X et al. "Analysis of miRNA expression profiles in melatonin-exposed GC-1 spg cell line." Gene :513-521 (2018). DOI: 10.1016/j.gene.2017.11.068. Application: PCR.