From RNA template to cDNA in just 15 minutes completely hassle-free. With abm’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience.
Key Features
• Reduction in handling errors with only 1 liquid transfer step
• Streamlined protocol suitable for high-throughput applications
• Simple set-up for any RNA template
• High reproducibility and excellent yield
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
Template
RNA
Format
MasterMix
Mix Concentration
5X
Quantity
25 x 10 µl reactions
Reaction
RT-PCR
Reference
Almousaa, AA et al. "Elevation of serum pyruvate kinase M2 (PKM2) in IBD and its relationship to IBD indices." Clin. Biochem. 17:30729-4 (2017). DOI: 10.1016/j.clinbiochem.2017.12.007. Application: cDNA synthesis.
An, L et al. "Salvia miltiorrhiza Lipophilic Fraction Attenuates Oxidative Stress in Diabetic Nephropathy through Activation of Nuclear Factor Erythroid 2-Related Factor 2." The American Joual of Chinese Medicine 7:1441-1457 (2017). DOI: 10.1142/S0192415X17500781. Application: qPCR.
Austin, K et al. "Insulin-like Growth Factor Binding Protein-4 is Required for the Growth Effects of Glucagon-like Peptide-2 In Murine Intestine.." Endocrinology 156(2):429-436 (2014). PubMed: 25514089. Application: Reverse Transcription.
Bradley, R et al. "Glucagon-like peptide-2 requires a full-complement of Bmi-1 for its proliferative effects in the murine small intestine." Endocrinology 7: (2016). DOI: http://dx.doi.org/10.1210/en.2016-1127 . Application: Reverse Transcription.
Chen, F et al. "Fine mapping identifes CsGCN5 encoding a histone acetyltransferase as putative candidate gene for mutation (td‑1) in cucumbertendril‑less1." Theor. Appl. Genet. :1549-1558 (2017). DOI: 10.1007/s00122-017-2909-1. Application: RT-PCR.
Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
Cui, C et al. "LAPTM4B-35 expression was correlated with favorable post-operation survival in pancreatic cancer patients." Translational Cancer Research 4:709-719 (2017). DOI: 10.21037/tcr.2017.07.11. Application: cDNA synthesis.
Dai, X et al. "Exteal Ca 2+ regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." Exp. Cell. Res. : (2016). DOI: http://dx.doi.org/10.1016/j.yexcr.2016.11.004. Application: Reverse Transcription.
Deng, H et al. "An efficient polyethylene glycol-mediated transformation system of lentiviral vector in Shiraia bambusicola." Process Biochemistry 10:1357-1362 (2016). DOI: http://dx.doi.org/10.1016/j.procbio.2016.07.013. Application: Reverse Transcription.
Deng, H et al. "Genome editing in Shiraia bambusicola using CRISPR-Cas9 system." J. Biotechnol. :228-234 (2017). DOI: 10.1016/j.jbiotec.2017.06.1204. Application: cDNA synthesis.
Deng, H et al. "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." Gene 1:67-72 (2016). DOI: http://dx.doi.org/10.1016/j.gene.2016.01.019. Application: Reverse Transcription.
Di, M et al. "Baseline value of intrahepatic HBV DNA over cccDNA predicts patient’s response to interferon therapy." Scientific Reports, a nature research joual : (2017). DOI: 10.1038/s41598-017-05242-y. Application: cDNA synthesis.
Gagnon, j et al. "Chronic Exposure to TNFα Impairs Secretion of Glucagon-Like Peptide-1." Endocrinology 156(11):3950-60 (2015). DOI: 10.1210/en.2015-1361. PubMed: 26270730. Application: Reverse Transcription.
Gagnon, J et al. "Ghrelin Is a Novel Regulator of GLP-1 Secretion." Diabetes 64(5):1513-21 (2015). DOI: 10.2337/db14-1176. PubMed: 25412624. Application: Reverse Transcription.
Gao, R et al. "Direct induction of neural progenitor cells transiently passes through a partially reprogrammed state." Biomaterials :53-67 (2017). DOI: 10.1016/j.biomaterials.2016.12.007. Application: RT-PCR.
Gordon, HM et al. "STEAP4 expression in human islets is associated with differences in body mass index, sex, HbA1c, and inflammation." Endocrine 3:528-537 (2017). DOI: 10.1007/s12020-017-1297-2. Application: RT-PCR.
Hanson, M et al. "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." BioMed Central 1:228 (2016). DOI: 10.1186/s12862-016-0805-y. Application: Reverse Transcription.
He, Y et al. "Differential immune-related gene expression in the spleens of duck Tembusu virus-infected goslings." Veterinary Microbiology :39-47 (2017). DOI: 10.1016/j.vetmic.2017.08.002. Application: cDNA synthesis.
Hou, W et al. "Clock gene affect the noncanonical NF-ĸB pathway via circadian variation of Otud7b." Biol. Rhythm Res. 6:939-950 (2017). DOI: 10.1080/09291016.2017.1323422. Application: RT-PCR.
Jeon, H.J et al. "Comparison of the functional characterization between alkane monooxygenases for low-molecular-weight polyethylene biodegradation." Int. Biodeterior. Biodegrad. :202-208 (2016). DOI: http://dx.doi.org/10.1016/j.ibiod.2016.06.012. Application: Reverse Transcription.
Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
Ju, M et al. "Dissecting the genetic architecture of Fusarium verticillioides seed rot resistance in maize by combining QTL mapping and genome-wide association analysis." Sci. Rep. :46446 (2017). DOI: 10.1038/srep46446. Application: qRT-PCR.
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Lei, M et al. "Gene cloning, expression, and characterization of trehalose-6-phosphate synthase from Pleurotus ostreatus." Joual of Basic Microbiology : (2017). DOI: 10.1002/jobm.201700120. Application: RT-PCR.
Li, L et al. "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." Int. Immunopharmacol. :189-198 (2016). DOI: http://dx.doi.org/10.1016/j.intimp.2016.02.019. Application: Reverse Transcription.
Li, Q et al. "Etanercept protects rat cardiomyocytes against hypertrophy by regulating inflammatory cytokines secretion and cell apoptosis." Brazilian Joual of Medical and Biological Research : (2017). DOI: 10.1590/1414-431x20175868 . Application: RT-PCR.
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Li, T et al. "Method for quantitative detection of FAM19A4 by flow cytometry using latex beads as solid carrier." J. Biosci. Bioeng. : (2017). DOI: 10.1016/j.jbiosc.2017.10.008. Application: RT-PCR.
Lin, et al. "Phosphorylation and negative regulation of CONSTITUTIVELY PHOTOMORPHOGENIC 1 by PINOID in Arabidopsis." PNAS 25:6617-6622 (2017). DOI: 10.1073/pnas.1702984114. Application: qRT-PCR.
Liu, F et al. "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING‐LIKE 1 (OsCOLE1) and OsCOLE1‐INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." New Phytologist 1:96-107 (2016). DOI: 10.1111/nph.14021. Application: Reverse Transcription.
Liu, J.C et al. "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." Inteational Joual of Cardiology :388-393 (2016). DOI: http://dx.doi.org/10.1016/j.ijcard.2016.11.202. Application: Reverse Transcription.
Liu, P et al. "Functional regulation of Q by microRNA172 and transcriptional co-repressor TOPLESS in controlling bread wheat spikelet density." Plant Biotechnol. J. :1-12 (2017). DOI: 10.1111/pbi.12790. Application: RT-PCR.
Martchenko, A et al. "Suppression of circadian secretion of glucagon-like peptide-1 by the saturated fatty acid, palmitate." Acta Physiol. : (2017). DOI: 10.1111/apha.13007. Application: RT-PCR.
Niu, B et al. "Transcriptome Sequencing Reveals Astrocytes as a Therapeutic Target in Heat-Stroke." Neuroscience Bulletin 6:627-640 (2017). DOI: 10.1007/s12264-017-0156-8. Application: Reverse Transcription.
Shi, Y et al. "Smad nuclear interacting protein 1 (SNIP1) inhibits intestinal inflammation through regulation of epithelial barrier function." Mucosal Immunology : (2017). DOI: 10.1038/mi.2017.95. Application: RT-PCR.
Su, X et al. "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." Int. Immunopharmacol. :16-25 (2016). DOI: http://dx.doi.org/10.1016/j.intimp.2016.05.005. Application: Reverse Transcription.
Sun, M et al. "Hypoxia inducible factor‐1α‐induced IL‐33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." Clinical & Experimental Immunology : (2016). DOI: 10.1111/cei.12896. Application: Reverse Transcription.
Tuerdi, N et al. "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." Nanomed. Nanotechnol. Biol. Med. 7:1899-1907 (2016). DOI: http://dx.doi.org/10.1016/j.nano.2016.05.002. Application: Reverse Transcription.
Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.
Wang, H et al. "The Cytochrome P450 Gene CsCYP85A1 Is a Putative Candidate for Super Compact-1 (Scp-1) Plant Architecture Mutation in Cucumber (Cucumis sativus L.)." Front. Plant Sci. :1-013 (2017). DOI: 10.3389/fpls.2017.00266. Application: RT-PCR.
Wu, R et al. "MicroRNA-448 promotes multiple sclerosis development through induction of Th17 response through targeting protein tyrosine phosphatase non-receptor type 2 (PTPN2)." Biochemial and Biophysical Research Commincations 3:759-766 (2017). DOI: 10.1016/j.bbrc.2017.03.115. Application: RT-PCR.
Xiong, Y et al. "ATF6 knockdown decreases apoptosis, arrests the S phase of the cell cycle, and increases steroid hormone production in mouse granulosa cells." American Joual of Cell Physiology 3:C341-C353 (2017). DOI: 10.1152/ajpcell.00222.2016. Application: qRT-PCR.
Yang, S et al. "Natural Infection of Avian Hepatitis E Virus and Marek's Disease Virus in Brown Layer Chickens in China." Avian Diseases 3:698-704 (2016). Application: Reverse Transcription.
Yang, W et al. "Critical role of ROCK2 activity in facilitating mucosal CD4þ T cell activation in inflammatory bowel disease." Joual of Autoimmunity 17:1-14 (2017). DOI: 10.1016/j.jaut.2017.12.009. Application: cDNA Synthesis.
Yu, M et al. "Bisphenol A accelerates meiotic progression in embryonic chickens via the estrogen receptor β signaling pathway." General and Comparative Endocrinology : (2017). DOI: 10.1016/j.ygcen.2017.11.004. Application: cDNA synthesis.
Zhang, M et al. "Transcription factors NF-YA2 and NF-YA10 regulate leaf growth via auxin signaling in Arabidopsis." Sci. Rep. 1:1395 (2017). DOI: 10.1038/s41598-017-01475-z. Application: RT-PCR.
Zheng, H et al. "The quorum sensing regulator CinR hierarchically regulates two other quorum sensing pathways in ligand-dependent and -independent fashions in Rhizobium etli." J Bacteriol 197(9):1573-81 (2015). DOI: 10.1128/JB.00003-15. PubMed: 25691531. Application: Reverse Transcription.
Zhong-yi, H et al. "Evaluation of vitrification protocol of mouse ovarian tissue by effect of DNA methyltransferase-1 and pateal imprinted growth factor receptor-binding protein 10 on signaling pathways." Cryobiology : (2017). DOI: 10.1016/j.cryobiol.2017.11.008.. Application: RT-PCR.
Zhou, G et al. ""Tripartite motif-containing (TRIM) 21 negatively regulates intestinal mucosal inflammation through inhibiting TH1/TH17 cell differentiation in patients with inflammatory bowel diseases"." J Allergy Clin Immunol. 17:31731-1 (2017). PubMed: 29113905. Application: qRT-PCR.
Zhou, H et al. "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." Scientific reports :25545 (2016). DOI: 10.1038/srep25545. Application: Reverse Transcription.
Zhou, H et al. "LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies." Frontiers in microbiology :166 (2016). DOI: 10.3389/fmicb.2016.00166. Application: Reverse Transcription.
Zhou, Q et al. "Identification of Type III Interferon (IFN-λ) in Chinese Goose: Gene Structure, Age-Dependent Expression Profile, and Antiviral Immune Characteristics In Vivo and In Vitro." Joual of Interderon & Cytokine Research 6:269-277 (2017). DOI: 10.1089/jir.2016.0061. Application: RT-PCR.
Zhou, R et al. "JNK Pathway-Associated Phosphatase/DUSP22 Suppresses CD4+ T-Cell Activation and Th1/Th17-Cell Differentiation and Negatively Correlates with Clinical Activity in Inflammatory Bowel Disease." Front. Immunol. 781: (2017). DOI: 10.3389/fimmu.2017.00781. Application: qRT-PCR.