From RNA template to cDNA in just 15 minutes completely hassle-free. With abm’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience.
Key Features
• Reduction in handling errors with only 1 liquid transfer step
• Streamlined protocol suitable for high-throughput applications
• Simple set-up for any RNA template
• High reproducibility and excellent yield
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
Template
RNA
Format
MasterMix
Quantity
200 x 10 µl reactions
Reaction
RT-PCR
Reference
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Deng, H et al. "An efficient polyethylene glycol-mediated transformation system of lentiviral vector in Shiraia bambusicola." Process Biochemistry 10:1357-1362 (2016). DOI: http://dx.doi.org/10.1016/j.procbio.2016.07.013. Application: Reverse Transcription.
Deng, H et al. "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." Gene 1:67-72 (2016). DOI: http://dx.doi.org/10.1016/j.gene.2016.01.019. Application: Reverse Transcription.
Gagnon, J et al. "Chronic Exposure to TNFα Impairs Secretion of Glucagon-Like Peptide-1." Endocrinology 156(11):3950-60 (2015). DOI: 10.1210/en.2015-1361. PubMed: 26270730. Application: Reverse Transcription.
Gagnon, J et al. "Ghrelin Is a Novel Regulator of GLP-1 Secretion." Diabetes 64(5):1513-21 (2015). DOI: 10.2337/db14-1176. PubMed: 25412624. Application: Reverse Transcription.
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Li, L et al. "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." Int. Immunopharmacol. :189-198 (2016). DOI: http://dx.doi.org/10.1016/j.intimp.2016.02.019. Application: Reverse Transcription.
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Liu, F et al. "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING‐LIKE 1 (OsCOLE1) and OsCOLE1‐INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." New Phytologist 1:96-107 (2016). DOI: 10.1111/nph.14021. Application: Reverse Transcription.
Liu, J.C et al. "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." Inteational Joual of Cardiology :388-393 (2016). DOI: http://dx.doi.org/10.1016/j.ijcard.2016.11.202. Application: Reverse Transcription.
Su, X et al. "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." Int. Immunopharmacol. :16-25 (2016). DOI: http://dx.doi.org/10.1016/j.intimp.2016.05.005. Application: Reverse Transcription.
Sun, M et al. "Hypoxia inducible factor‐1α‐induced IL‐33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." Clinical & Experimental Immunology : (2016). DOI: 10.1111/cei.12896. Application: Reverse Transcription.
Tang, X et al. "Doxycycline attenuates breast cancer related inflammation by decreasing plasma lysophosphatidate concentrations and inhibiting NF-κB activation." Molecular Cancer 36: (2017). DOI: 10.1186/s12943-017-0607-x. Application: RT .
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Yang, S et al. "Natural Infection of Avian Hepatitis E Virus and Marek's Disease Virus in Brown Layer Chickens in China." Avian Diseases 3:698-704 (2016). Application: Reverse Transcription.
Zheng, H et al. "The quorum sensing regulator CinR hierarchically regulates two other quorum sensing pathways in ligand-dependent and -independent fashions in Rhizobium etli." J Bacteriol 197(9):1573-81 (2015). DOI: 10.1128/JB.00003-15. PubMed: 25691531. Application: Reverse Transcription.
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