PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 19 bands, ranging from 100 pb – 10 kb, for molecular weight standards in agarose gel electrophoresis. The 500, 1,500, and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
Suggested-load
Resolves into 19 sharp, tight bands.Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.
Bands
19 bands
Quantity
500 µl/100 loads
Range
100bp-10kb
Reaction
Marker
Reference
Gnanandarajah, JS et al. "Identification by mass spectrometry and immune response analysis of guinea pig cytomegalovirus (GPCMV) pentameric complex proteins GP129, 131 and 133." Viruses 6 (2):727-751 (2014). DOI: 10.3390/v6020727. PubMed: 24531333. Application: PCR Products size reference.