JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Primary Antibodies

p44/42 MAPK (Erk1/2) Antibody

SKU : BHA12901207

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

AFFINITY BIOSCIENCES LTD

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	BF0412
Alternative Names	ERK 1 ; ERK ; ERK-1 ; ERK1 ; ERT 2 ; ERT2 ; Extracellular Signal Regulated Kinase 1 ; Extracellular signal related kinase 1 ; Extracellular signal-regulated kinase 1 ; HGNC6877 ; HS44KDAP ; HUMKER1A ; Insulin Stimulated MAP2 Kinase ; Insulin-stimulated MAP2 kinase ; MAP kinase 1 ; MAP kinase 3 ; MAP Kinase ; MAP kinase isoform p44 ; MAPK 1 ; MAPK 3 ; MAPK ; MAPK1 ; Mapk3 ; MGC20180 ; Microtubule Associated Protein 2 Kinase ; Microtubule-associated protein 2 kinase ; Mitogen Activated Protein Kinase 3 ; Mitogen-activated protein kinase 1 ; Mitogen-activated protein kinase 3 ; MK03_HUMAN ; OTTHUMP00000174538 ; OTTHUMP00000174541 ; p44 ERK1 ; p44 MAPK ; p44-ERK1 ; p44-MAPK ; P44ERK1 ; P44MAPK ; PRKM 3 ; PRKM3 ; Protein Kinase Mitogen Activated 3 ; ERK 2 ; ERK ; ERK-2 ; ERT1 ; Extracellular Signal Regulated Kinase 2 ; Extracellular signal-regulated kinase 2 ; MAP kinase 1 ; MAP kinase 2 ; MAP kinase isoform p42 ; MAPK 1 ; MAPK 2 ; Mapk1 ; MAPK2 ; Mitogen-activated protein kinase 1 ; Mitogen-activated protein kinase 2 ; MK01_HUMAN ; P38 ; P40 ; P41 ; p42-MAPK ; P42MAPK ; PRKM1 ; PRKM2 ; protein kinase, mitogen-activated, 1 ; protein kinase, mitogen-activated, 2 ; protein tyrosine kinase ERK2 ;
Categories	Primary Antibodies
Clonality	monoclonal
Description	Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.
Immunogen	Purified recombinant fragment of human p44/42 MAPK (Erk1/2) expressed in E. Coli.
Molecular Weight	Observed Mol.Wt.: 42kDa. Predicted Mol.Wt.: 44kDa.
Reactivity	Human, Mouse
Specificity	p44/42 MAPK (Erk1/2) antibody detects endogenous levels of total p44/42 MAPK (Erk1/2).
Subcellular Location	Nucleus.

Uniprot

P27361 ; P28482

Gene Id

55955594

Sequence

10	20	30	40	50
MAAAAAQGGG	GGEPRRTEGV	GPGVPGEVEM	VKGQPFDVGP	RYTQLQYIGE
60	70	80	90	100
GAYGMVSSAY	DHVRKTRVAI	KKISPFEHQT	YCQRTLREIQ	ILLRFRHENV
110	120	130	140	150
IGIRDILRAS	TLEAMRDVYI	VQDLMETDLY	KLLKSQQLSN	DHICYFLYQI
160	170	180	190	200
LRGLKYIHSA	NVLHRDLKPS	NLLINTTCDL	KICDFGLARI	ADPEHDHTGF
210	220	230	240	250
LTEYVATRWY	RAPEIMLNSK	GYTKSIDIWS	VGCILAEMLS	NRPIFPGKHY
260	270	280	290	300
LDQLNHILGI	LGSPSQEDLN	CIINMKARNY	LQSLPSKTKV	AWAKLFPKSD
310	320	330	340	350
SKALDLLDRM	LTFNPNKRIT	VEEALAHPYL	EQYYDPTDEP	VAEEPFTFAM
360	370
ELDDLPKERL	KELIFQETAR	FQPGVLEAP

Concentration	1mg/ml
Format	Liquid
Purification	Affinity-chromatography.
Storage Condition	Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	ELISA, IHC, FACS, WB
Dilution	ELISA 1/10000, WB 1/500 - 1/2000, IHC 1/200 - 1/1000, FCM 1/200 - 1/400, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
IHC Protocol.
Western Blotting Handbook.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHA12901207	BF0412	100ul	$350		Add to Cart
BHA12901207	BF0412	200ul	$450		Add to Cart
BHA12901207	BF0412	50ul	$250		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.