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Home / Assay Kits

NanoGram SDS Assay Kit

SKU : BHE16300269

This product includes 20 ml of MPS6 Dye. It is for measurement of 200 samples using 96-well plates. Cuvettes may also be used for measurements.

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Mfr.No.	SDS200
Description	SDS (sodium dodecyl sulfate or sodium lauryl sulfate) is a common detergent and protein denaturant. In laboratories, SDS is broadly used in cell lysis, protein analysis (SDS-PAGE) and membrane protein folding studies. In industry, SDS is used as a highly effective surfactant and is a key component in many cleaning products. SDS strongly binds to proteins and changes the protein conformation that affects the protein function. Complete removal of SDS in a protein solution is essential to fully recover the protein function and stability. The NanoGram SDS Assay Kit (Catalog number SDS200) is designed for measurement of nanograms of SDS. The assay sensitivity is 0.002% SDS which is about 100 fold lower than its CMC value. The assay is based on increase of light absorbance at 650 nm of the dye MPS6 in the presence of SDS. The assay kit can be used for measurements SDS concentrations in samples with or without proteins. The assay is compatible with most biochemical buffers. It is not compatible with phosphate buffers. Inorganic phosphate interferes with the assay. The NanoGram SDS Assay Kit (catalog number SDS200) includes 20 ml of MPS6 Dye. It is for measurement of 200 samples using 96-well plates. Cuvettes may also be used for measurements.
Concentration measurement	Detergent and lipids

Product Manual	Datasheet
Protocols Description	ASSAY PROTOCOL The following assay protocol is based on using a 96-well plate for the measurement. The sample volume is 100 µl and the final assay volume is 200 µl. For 384-well plate assays, the sample volume is 40 µl and the final assay volume is 80 µl. For assays using cuvette, the sample volume is 500 µl and the final assay volume is 1000 µl. STANDARD CURVE 1. Sample preparation: Prepare 100 µl of SDS solutions in the wells of a transparent 96-well plate with a two-fold serial dilution from 0.05% to zero in water or a non-phosphate buffer. 2. Detection: Mix 100 µl of Dye MPS6 with 100 µl of the SDS solutions for 5 min and read the light absorbance at 650 nm (OD₆₅₀). 3. Data Analysis: Plot the OD₆₅₀ values with the SDS concentration [SDS] to generate the linear standard curve. OD₆₅₀ = a [SDS] + b Where the OD₆₅₀ values are from experimental data, the a and b values are from the linear fitting between the OD₆₅₀ values and the SDS concentrations. UNKNOWN SAMPLES Follow the same procedure to measure the OD₆₅₀ values from the unknown samples. Calculate the SDS concentrations in the unknown samples using the OD₆₅₀ values from the unknown samples and the a and b values from the standard curve. [SDS] = (OD₆₅₀ – b) / a

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300269	SDS200	200 assays	$302.17		Add to Cart

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