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Home / Proteins & Peptide

mTOR/Raptor/mLST8 Complex, FLAG-tag, His-tag

SKU : BHP18200220

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BPS Bioscience

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Mfr.No.	40300
Description	Complex of human mTOR, also known as FRAP1 (GenBank Accession No. NM_004958), a.a. 1362-end, with N-terminal FLAG-tag, MW = 137 kDa, human MLST8 (GenBank Accession No. NM_022372), a.a. 2-326 (end) with N-terminal His-tag, MW = 37 kDa, and human Raptor (GenBank Accession No. NM_020761), a.a. 2-1335 (end) with N-terminal Histag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 7.1 pmol/min/ug
Immunogen Region	mTOR 1362-end, MLST8 2-326 (end), Raptor 2-1335 (end)
Tag	N-terminal FLAG-tag (mTOR), N-terminal His-tag (MLST8), N-terminal His-tag (Raptor)
Formulation	40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/uL FLAG peptide, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35 and 2 uM substrate (Invitrogen Zlyte Ser/Thr peptide 11) at room temperature for 1 hour. Avoid freeze/thaw cycles.
GenBank	NM_004958 (mTOR), NM_022372 (MLST8), NM_020761 (Raptor)
Mw(kda)	324 kDa (complex)

Synonyms	mTOR, mechanistic target of rapamycin, FRAP, FKBP-Rapamycin Associated Protein, Mammalian Target Of Rapamycin, raptor, regulatory associated protein of MTOR, Mip1, MLST8, Mammalian Lethal With SEC13 Protein 8, GBL, Gable, POP3, LST8
Uniprot	P42345 (mTOR), Q9BVC4 (MLST8), Q8N122 (Raptor)

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Toral-Barza et al., (2005) Biochem. Biophys. Res. Commun. 24, 332(1):304-10. 2. Rubio-Viqueira and Hidalgo, M. (2006) Adv. Exp. Med. Biol. 587:309-327.Application Reference(s): 1. mTOR signaling regulates nucleolar targeting of the SUMO-specific isopeptidase SENP3 (2014) 2.The mechanistic target for rapamycin pathway is related to the phosphorylation score for estrogen receptor-alpha in human breast tumors in vivo (2014)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200220	40300	10 ug	$511.50		Add to Cart

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